Recombinant Mouse Gba2 Protein, Myc/DDK-tagged

• Cat.No.: Gba2-3166M
• Product Overview: Purified recombinant protein of mouse full-length glucosidase beta 2 (Gba2), with C-terminal MYC/DDK tag, expressed in HEK293T cells.

Specification

• Description: Non-lysosomal glucosylceramidase that catalyzes the hydrolysis of glucosylceramide (GlcCer) to free glucose and ceramide. Glucosylceramides are membrane glycosphingolipids that have a wide intracellular distribution. They are the main precursors of more complex glycosphingolipids that play a role in cellular growth, differentiation, adhesion, signaling, cytoskeletal dynamics and membrane properties. Also involved in the transglucosylation of cholesterol, transferring glucose from glucosylceramides, thereby modifying its water solubility and biological properties. Under specific conditions, may catalyze the reverse reaction, transferring glucose from cholesteryl-beta-D-glucoside to ceramide. Finally, may also play a role in the metabolism of bile acids. It is able to hydrolyze bile acid 3-O-glucosides but also to produce bile acid-glucose conjugates thanks to a bile acid glucosyl transferase activity. However, the relevance of both activities is unclear in vivo.
• Source: HEK293T
• Species: Mouse
• Tag: Myc/DDK
• Molecular Mass: 103.7 kDa
• Purity: > 80% as determined by SDS-PAGE and Coomassie blue staining
• Stability: Stable for 12 months from the date of receipt of the product under proper storage and handling conditions. Avoid repeated freeze-thaw cycles.
• Storage: Store at -80 centigrade after receiving vials.
• Concentration: >50 μg/mL as determined by microplate BCA method
• Storage Buffer: 25 mM Tris.HCl, pH 7.3, 100 mM glycine, 10% glycerol.

Gene Information

• Gene Name: Gba2 glucosidase beta 2 [ Mus musculus (house mouse) ]
• Official Symbol: Gba2
• Synonyms: Gba2; glucosidase beta 2; F630034E04; non-lysosomal glucosylceramidase; NLGase; beta-glucocerebrosidase 2; beta-glucosidase 2; bile acid beta-glucosidase GBA2; bile acid glucosyl transferase GBA2; cholesterol glucosyltransferase GBA2; cholesteryl-beta-glucosidase GBA2; glucosidase, beta (bile acid) 2; glucosylceramidase 2; EC 3.2.1.45
• Gene ID: 230101
• mRNA Refseq: NM_172692
• Protein Refseq: NP_766280
• UniProt ID: Q69ZF3