Recombinant Mouse Gckr Protein, Myc/DDK-tagged

• Cat.No.: Gckr-3174M
• Product Overview: Purified recombinant protein of mouse full-length glucokinase regulatory protein (Gckr), with C-terminal MYC/DDK tag, expressed in HEK293T cells.

Specification

• Species: Mouse
• Source: HEK293
• Tag: DDK&Myc
• Description: Regulates glucokinase (GCK) by forming an inactive complex with this enzyme. Acts by promoting GCK recruitment to the nucleus, possibly to provide a reserve of GCK that can be quickly released in the cytoplasm after a meal. The affinity of GCKR for GCK is modulated by fructose metabolites: GCKR with bound fructose 6-phosphate has increased affinity for GCK, while GCKR with bound fructose 1-phosphate has strongly decreased affinity for GCK and does not inhibit GCK activity.
• Molecular Mass: 65.1 kDa
• Purity: > 80% as determined by SDS-PAGE and Coomassie blue staining
• Stability: Stable for 12 months from the date of receipt of the product under proper storage and handling conditions. Avoid repeated freeze-thaw cycles.
• Storage: Store at -80 centigrade after receiving vials.
• Concentration: >50 μg/mL as determined by microplate BCA method
• Storage Buffer: 25 mM Tris.HCl, pH 7.3, 100 mM glycine, 10% glycerol.

Gene Information

• Gene Name: Gckr glucokinase regulatory protein [ Mus musculus (house mouse) ]
• Official Symbol: Gckr
• Synonyms: Gckr; glucokinase regulatory protein; GK; GKRP; glucokinase regulatory protein; glucokinase regulator
• Gene ID: 231103
• mRNA Refseq: NM_144909
• Protein Refseq: NP_659158
• UniProt ID: Q91X44