Recombinant Mouse Gpc3 Protein, hFc-tagged, Alexa Fluor 488 conjugated

• Cat.No.: Gpc3-120MAF488
• Product Overview: Alexa Fluor 488 conjugated recombinant Mouse Gpc3 protein (Gln25-Met557), fused to human IgG1 Fc tag at C-terminus, was expressed in human 293 cells (HEK293).

Specification

• Species: Mouse
• Source: HEK293
• Tag: Fc
• Protein Length: 533
• Description: Glypican-3 (GPC3) is also known as Intestinal protein OCI-5, GTR2-2, MXR7, which belongs to the glypican family. Glypican 3/GPC-3 is highly expressed in lung, liver and kidney. Glypican-3 inhibits the dipeptidyl peptidase activity of DPP4. Glypican-3 may be involved in the suppression/modulation of growth in the predominantly mesodermal tissues and organs, and also may play a role in the modulation of IGF2 interactions with its receptor and thereby modulate its function.
• Form: Lyophilized
• Molecular Mass: This protein contains a furin-like convertase cleavage site, 354-RQYR-357, and will be partially processed into N and C-terminal fragment with calculated MW of 38.0 kDa and 49.1 kDa respectively. The protein migrates as 40 kDa, 60 kDa and 100 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.
• Endotoxin: < 1.0 EU/ μg by the LAL method.
• Purity: > 90 % GPC3 and 2-8 % human IgG1 Fc fragment as determined by SDS-PAGE.
• Characteristic: Disulfide-linked homodimer
  Labeled with Alexa Fluor 488 via amines
  Excitation Wavelength: 488 nm
  Emission Wavelength: 515­-545 nm
• Storage: For long term storage, the product should be stored at lyophilized state at -20 centigrade or lower.
  Please avoid repeated freeze-thaw cycles.
  This product is stable after storage at:
  -20 to -70 centigrade for 12 months in lyophilized state;
  -70 centigrade for 3 months under sterile conditions after reconstitution.
• Storage Buffer: Lyophilized from 0.22 μm filtered solution in Tris with Glycine, Arginine and NaCl, pH7.5, 10% trehalose.
• Reconstitution: It is recommended that sterile water be added to the vial to prepare a stock solution of 0.2 μg/μL. Centrifuge the vial at 4 centigrade before opening to recover the entire contents.
• Conjugation: Alexa Fluor 488

Gene Information

• Gene Name: Gpc3
• Official Symbol: Gpc3
• Synonyms: GPC3; glypican 3; glypican-3; OCI-5
• Gene ID: 14734
• mRNA Refseq: NM_016697
• Protein Refseq: NP_057906
• UniProt ID: Q8CFZ4