Recombinant Mouse Gskip Protein, Myc/DDK-tagged

• Cat.No.: Gskip-3319M
• Product Overview: Purified recombinant protein of mouse full-length GSK3B interacting protein (Gskip), with C-terminal MYC/DDK tag, expressed in HEK293T cells.

Specification

• Description: A-kinase anchoring protein for GSK3B and PKA that regulates or facilitates their kinase activity towards their targets. The ternary complex enhances Wnt-induced signaling by facilitating the GSK3B- and PKA-induced phosphorylation of beta-catenin leading to beta-catenin degradation and stabilization respectively. Upon cAMP activation, the ternary complex contributes to neuroprotection against oxidative stress-induced apoptosis by facilitating the PKA-induced phosphorylation of DML1 and PKA-induced inactivation of GSK3B. During neurite outgrowth promotes neuron proliferation; while increases beta-catenin-induced transcriptional activity through GSK3B kinase activity inhibition, reduces N-cadherin level to promote cell cycle progression. May play a role in cleft palate formation and is required for postnatal life through modulation of the activity of GSK3B during development.
• Source: HEK293T
• Species: Mouse
• Tag: Myc/DDK
• Molecular Mass: 16.2 kDa
• Purity: > 80% as determined by SDS-PAGE and Coomassie blue staining
• Stability: Stable for 12 months from the date of receipt of the product under proper storage and handling conditions. Avoid repeated freeze-thaw cycles.
• Storage: Store at -80 centigrade after receiving vials.
• Concentration: >50 μg/mL as determined by microplate BCA method
• Storage Buffer: 25 mM Tris.HCl, pH 7.3, 100 mM glycine, 10% glycerol.

Gene Information

• Gene Name: Gskip GSK3B interacting protein [ Mus musculus (house mouse) ]
• Official Symbol: Gskip
• Synonyms: Gskip; GSK3B interacting protein; 4933433P14Rik; GSK3B-interacting protein; GSK3-beta interaction protein
• Gene ID: 66787
• mRNA Refseq: NM_178613
• Protein Refseq: NP_848728
• UniProt ID: Q8BGR8