Recombinant Mouse Gtf2b Protein, Myc/DDK-tagged

• Cat.No.: Gtf2b-3323M
• Product Overview: Purified recombinant protein of mouse full-length general transcription factor IIB (Gtf2b), with C-terminal MYC/DDK tag, expressed in HEK293T cells.

Specification

• Description: General transcription factor that plays a role in transcription initiation by RNA polymerase II (Pol II). Involved in the pre-initiation complex (PIC) formation and Pol II recruitment at promoter DNA. Together with the TATA box-bound TBP forms the core initiation complex and provides a bridge between TBP and the Pol II-TFIIF complex. Released from the PIC early following the onset of transcription during the initiation and elongation transition and reassociates with TBP during the next transcription cycle. Associates with chromatin to core promoter-specific regions. Binds to two distinct DNA core promoter consensus sequence elements in a TBP-independent manner; these IIB-recognition elements (BREs) are localized immediately upstream (BREu), 5'-[GC][GC][GA]CGCC-3', and downstream (BREd), 5'-[GA]T[TGA][TG][GT][TG][TG]-3', of the TATA box element. Modulates transcription start site selection. Exhibits also autoacetyltransferase activity that contributes to the activated transcription.
• Source: HEK293T
• Species: Mouse
• Tag: Myc/DDK
• Molecular Mass: 34.8 kDa
• Purity: > 80% as determined by SDS-PAGE and Coomassie blue staining
• Stability: Stable for 12 months from the date of receipt of the product under proper storage and handling conditions. Avoid repeated freeze-thaw cycles.
• Storage: Store at -80 centigrade after receiving vials.
• Concentration: >50 μg/mL as determined by microplate BCA method
• Storage Buffer: 25 mM Tris.HCl, pH 7.3, 100 mM glycine, 10% glycerol.

Gene Information

• Gene Name: Gtf2b general transcription factor IIB [ Mus musculus (house mouse) ]
• Official Symbol: Gtf2b
• Synonyms: GTF2B; general transcription factor IIB; transcription initiation factor IIB; general transcription factor TFIIB; RNA polymerase II alpha initiation factor; MGC6859
• Gene ID: 229906
• mRNA Refseq: NM_145546
• Protein Refseq: NP_663521
• UniProt ID: P62915