Recombinant Mouse Gtf3a Protein, His&GST-tagged

• Cat.No.: Gtf3a-1605M
• Product Overview: Recombinant Mouse Gtf3a Protein (Gly166-Cys364) with N-His&GST tag was expressed in E. coli.

Specification

• Description: The product of this gene is a zinc finger protein with nine Cis[2]-His[2] zinc finger domains. It functions as an RNA polymerase III transcription factor to induce transcription of the 5S rRNA genes. The protein binds to a 50 bp internal promoter in the 5S genes called the internal control region (ICR), and nucleates formation of a stable preinitiation complex. This complex recruits the TFIIIC and TFIIIB transcription factors and RNA polymerase III to form the complete transcription complex. The protein is thought to be translated using a non-AUG translation initiation site in mammals based on sequence analysis, protein homology, and the size of the purified protein.
• Source: E. coli
• Species: Mouse
• Tag: N-His&GST
• Form: Freeze-dried powder
• Molecular Mass: Predicted Molecular Mass: 52.9 kDa
  Accurate Molecular Mass: 53 kDa
• Protein length: Gly166-Cys364
• Purity: > 90%
• Applications: Positive Control; Immunogen; SDS-PAGE; WB.
• Stability: The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37 centigrade for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.
• Storage: Avoid repeated freeze/thaw cycles. Store at 2-8 centigrade for one month. Aliquot and store at -80 centigrade for 12 months.
• Storage Buffer: PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
• Reconstitution: Reconstitute in PBS or others.

Gene Information

• Gene Name: Gtf3a general transcription factor III A [ Mus musculus (house mouse) ]
• Official Symbol: Gtf3a
• Synonyms: GTF3A; general transcription factor III A; transcription factor IIIA; TFIIIA; factor A; 2010015D03Rik; 2610111I01Rik; 5330403M05Rik
• Gene ID: 66596
• mRNA Refseq: NM_025652
• Protein Refseq: NP_079928
• UniProt ID: Q8VHT7