Recombinant Mouse Hace1 Protein, Myc/DDK-tagged

• Cat.No.: Hace1-3350M
• Product Overview: Purified recombinant protein of mouse full-length HECT domain and ankyrin repeat containing, E3 ubiquitin protein ligase 1 (Hace1), with C-terminal MYC/DDK tag, expressed in HEK293T cells.

Specification

• Description: E3 ubiquitin-protein ligase involved in Golgi membrane fusion and regulation of small GTPases. Acts as a regulator of Golgi membrane dynamics during the cell cycle: recruited to Golgi membrane by Rab proteins and regulates postmitotic Golgi membrane fusion. Acts by mediating ubiquitination during mitotic Golgi disassembly, ubiquitination serving as a signal for Golgi reassembly later, after cell division. Specifically interacts with GTP-bound RAC1, mediating ubiquitination and subsequent degradation of active RAC1, thereby playing a role in host defense against pathogens. May also act as a transcription regulator via its interaction with RARB.
• Source: HEK293T
• Species: Mouse
• Tag: Myc/DDK
• Molecular Mass: 102.1 kDa
• Purity: > 80% as determined by SDS-PAGE and Coomassie blue staining
• Stability: Stable for 12 months from the date of receipt of the product under proper storage and handling conditions. Avoid repeated freeze-thaw cycles.
• Storage: Store at -80 centigrade after receiving vials.
• Concentration: >50 μg/mL as determined by microplate BCA method
• Storage Buffer: 25 mM Tris.HCl, pH 7.3, 100 mM glycine, 10% glycerol.

Gene Information

• Gene Name: Hace1 HECT domain and ankyrin repeat containing, E3 ubiquitin protein ligase 1 [ Mus musculus (house mouse) ]
• Official Symbol: Hace1
• Synonyms: HACE1; HECT domain and ankyrin repeat containing, E3 ubiquitin protein ligase 1; E3 ubiquitin-protein ligase HACE1; HECT domain and ankyrin repeat-containing E3 ubiquitin-protein ligase 1; BC025474; 1700042J16Rik; A730034A22Rik; KIAA1320
• Gene ID: 209462
• mRNA Refseq: NM_172473
• Protein Refseq: NP_766061
• UniProt ID: Q3U0D9