Recombinant Mouse Hscb Protein, MYC/DDK-tagged

• Cat.No.: Hscb-169M
• Product Overview: Purified recombinant protein of full-length mouse HscB iron-sulfur cluster co-chaperone (Hscb), with C-terminal MYC/DDK tag, expressed in HEK293T cells.

Specification

• Description: Acts as a co-chaperone in iron-sulfur cluster assembly in both mitochondria and the cytoplasm. Required for incorporation of iron-sulfur clusters into SDHB, the iron-sulfur protein subunit of succinate dehydrogenase that is involved in complex II of the mitochondrial electron transport chain. Recruited to SDHB by interaction with SDHAF1 which first binds SDHB and then recruits the iron-sulfur transfer complex formed by HSC20, HSPA9 and ISCU through direct binding to HSC20. Also mediates complex formation between components of the cytosolic iron-sulfur biogenesis pathway and the CIA targeting complex composed of CIAO1, DIPK1B/FAM69B and MMS19 by binding directly to the scaffold protein ISCU and to CIAO1. This facilitates iron-sulfur cluster insertion into a number of cytoplasmic and nuclear proteins including POLD1, ELP3, DPYD and PPAT.
• Source: HEK293T
• Species: Mouse
• Tag: MYC/DDK
• Molecular Mass: 18.5 kDa
• Purity: >80%, as determined by SDS-PAGE and Coomassie blue staining
• Stability: Stable for 12 months from the date of receipt of the product under proper storage and handling conditions. Avoid repeated freeze-thaw cycles.
• Storage: Store at -80 centigrade after receiving vials.
• Concentration: >50 ug/mL as determined by microplate BCA method
• Storage Buffer: 25 mM Tris.HCl, pH 7.3, 100 mM glycine, 10% glycerol.

Gene Information

• Gene Name: Hscb HscB iron-sulfur cluster co-chaperone [ Mus musculus (house mouse) ]
• Official Symbol: Hscb
• Synonyms: Hscb; HscB iron-sulfur cluster co-chaperone; Hsc20; AI325508; AW049829; iron-sulfur cluster co-chaperone protein HscB; HscB iron-sulfur cluster co-chaperone homolog; J-type co-chaperone HSC20; iron-sulfur cluster co-chaperone protein HscB, mitochondrial
• Gene ID: 100900
• mRNA Refseq: NM_153571
• Protein Refseq: NP_705799
• UniProt ID: Q8K3A0