Recombinant Mouse HTRA1 Protein (141-480 aa), His-tagged

• Cat.No.: HTRA1-2578M
• Product Overview: Recombinant Mouse HTRA1 Protein (141-480 aa) is produced by E. coli expression system. This protein is fused with a 6xHis tag at the N-terminal. Research Area: Signal Transduction. Protein Description: Partial.

Specification

• Description: Serine protease with a variety of targets, including extracellular matrix proteins such as fibronectin. HTRA1-generated fibronectin fragments further induce synovial cells to up-regulate MMP1 and MMP3 production. May also degrade proteoglycans, such as aggrecan, decorin and fibromodulin. Through cleavage of proteoglycans, may release soluble FGF-glycosaminoglycan complexes that promote the range and intensity of FGF signals in the extracellular space. Regulates the availability of insulin-like growth factors (IGFs) by cleaving IGF-binding proteins. Inhibits signaling mediated by TGF-beta family members. This activity requires the integrity of the catalytic site, but it is unclear whether it leads to the proteolytic degradation of TGF-beta proteins themselves or not. By acting on TGF-beta signaling, may regulate many physiological processes, including retinal angiogenesis and neuronal survival and maturation during development. Intracellularly, degrades TSC2, leading to the activation of TSC2 downstream targets.
• Source: E. coli
• Species: Mouse
• Tag: His
• Form: Tris-based buffer,50% glycerol
• Molecular Mass: 39.0 kDa
• Protein length: 141-480 aa
• AA Sequence: KLRQPPVIVLQRGACGQGQEDPNSL RHKYNFIADVVEKIAPAVVHIELYR KLPFSKREVPVASGSGFIVSEDGLI VTNAHVVTNKNRVKVELKNGATYEA KIKDVDEKADIALIKIDHQGKLPVL LLGRSSELRPGEFVVAIGSPFSLQN TVTTGIVSTTQRGGKELGLRNSDMD YIQTDAIINYGNSGGPLVNLDGEVI GINTLKVTAGISFAIPSDKIKKFLT ESHDRQAKGKAVTKKKYIGIRMMSL TSSKAKELKDRHRDFPDVLSGAYII EVIPDTPAEAGGLKENDVIISINGQ SVVTANDVSDVIKKENTLNMVVRRG NEDIVITVIPEEIDP
• Purity: > 85% as determined by SDS-PAGE.
• Notes: Repeated freezing and thawing is not recommended. Store working aliquots at 4 centigrade for up to one week.
• Storage: The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself. Generally, the shelf life of liquid form is 6 months at -20 centigrade/-80 centigrade. The shelf life of lyophilized form is 12 months at -20 centigrade/-80 centigrade.
• Concentration: A hardcopy of COA will be sent along with the products. Please refer to it for detailed information.

Gene Information

• Gene Name: Htra1 HtrA serine peptidase 1 [ Mus musculus ]
• Official Symbol: HTRA1
• Synonyms: HTRA1; HtrA serine peptidase 1; serine protease HTRA1; serine protease 11; L56; HTRA; Prss11; RSPP11; AI429470
• Gene ID: 56213
• mRNA Refseq: NM_019564
• Protein Refseq: NP_062510
• UniProt ID: Q9R118