Active Recombinant Mouse Ido1 Protein, His-tagged

• Cat.No.: Ido1-7432M
• Product Overview: Recombinant Mouse Ido1(Ala2-Pro407) fused with an N-terminal Met and 6-His tag was expressed in E. coli.

Specification

• Description: Indoleamine 2,3-dioxygenase (IDO) is a heme-containing intracellular dioxygenase that catalyzes the degradation of the essential amino acid L-tryptophan to N formyl kynurenine. This degradation is the first and rate-limiting step of the L-kynurenine pathway. IDO expression is induced in macrophages, plasmacytoid dendritic cells (pDC), and microglia by multiple inflammatory stimuli including IFN-g, TNF-a, CpG DNA, HIV-1 Tat protein, microbial infection, or GITR Ligand activation. It can be expressed by tumor cells, pDC in tumor draining lymph nodes, and MDSC in breast cancer. IDO expression is critical for the ability of these cells to induce the activation of resting Treg and the support of immune tolerance to apoptotic cell debris. These cells also suppress anti-tumor immunity, the development of autoimmunity, and the replication of Chlamydia, Herpes simplex virus, and measles virus. In the absence of IDO function, activation of pDC does not induce immunosuppression but instead triggers IL-6 production and the redirecting of Treg to produce IL-17. Mouse IDO shares 61% and 87% amino acid sequence identity with human and rat IDO, respectively.
• Source: E. coli
• Species: Mouse
• Tag: His
• Predicted N Terminal: Met
• Form: Supplied as a 0.2 μm filtered solution in Sodium Acetate, NaCl and Glycerol.
• Bio-activity: Measured by its ability to oxidize L-tryptophan to N-formyl-kynurenine. The specific activity is >1,000 pmol/min/μg.
• Molecular Mass: redicted Molecular Mass: 46 kDa;
  SDS-PAGE: 41 kDa, reducing conditions.
• Protein length: Ala2-Pro407
• Endotoxin: <1.0 EU per 1 μg of the protein by the LAL method.
• Purity: >90%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  6 months from date of receipt, -20 to -70 centigrade as supplied.
  3 months, -20 to -70 centigrade under sterile conditions after opening.

Gene Information

• Gene Name: Ido1 indoleamine 2,3-dioxygenase 1 [ Mus musculus ]
• Official Symbol: Ido1
• Synonyms: IDO1; indoleamine 2,3-dioxygenase 1; IDO-1; indole 2,3-dioxygenase; indoleamine-pyrrole 2,3 dioxygenase; indoleamine-pyrrole 2,3-dioxygenase; Ido; Indo
• Gene ID: 15930
• mRNA Refseq: NM_008324
• Protein Refseq: NP_032350
• UniProt ID: P28776