Active Recombinant Mouse Ifng protein, His-tagged

• Cat.No.: Ifng-532M
• Product Overview: Active Recombinant Mouse Ifng protein(P01580)(23~155 aa), fused with N-terminal His tag, was expressed in E.coli.

Specification

• Species: Mouse
• Source: E.coli
• Tag: His
• Protein Length: 23-155 aa
• Form: Lyophilized from sterile 20mM Tris, 150mM NaCl, pH8.0, containing 1mM EDTA, 1mM DTT, 0.01% SKL, 5% Trehalose and Proclin300.
• Bio-activity: IFN-γ is an important activator of macrophages, it promotes production of inducible Nitric Oxide Synthase (iNOS) in macrophages. After stimulated with IFN-γ, morphological changes will occur in murine macrophage cell line (Raw 246.7 cells), and inducible nitric-oxide synthase (iNOS) in the cells will increase. Raw 246.7 cells were incubated in DMEM with IFN-γ (2ng/mL) for 24h, then cells were observed by inverted microscope and iNOS in cell lysates was detected by ELISA.
• Molecular Mass: 16.8kDa
• Endotoxin: <1.0EU per 1μg (determined by the LAL method).
• Purity: > 95% as determined by SDS-PAGE.
• Storage: Avoid repeated freeze/thaw cycles. Store at -20°C for 12 months. Aliquot and store at -80°C for 12 months.
• Reconstitution: Reconstitute in 10mM PBS (pH7.4) to a concentration of 0.1-1.0 mg/mL. Do not vortex.

Gene Information

• Gene Name: Ifng interferon gamma [ Mus musculus ]
• Official Symbol: Ifng
• Synonyms: IFNG; interferon gamma; IFN-gamma; gamma interferon; Ifg; IFN-g
• Gene ID: 15978
• mRNA Refseq: NM_008337
• Protein Refseq: NP_032363