"IL12" Related Products


Recombinant Mouse IL12 protein, His-tagged

Cat.No.: IL12-28M
Product Overview: This product contains IL12A & IL12B heterodimer. A DNA sequence encoding the p35 subunit of mouse IL12, termed as IL12A (P43431) (Met 1-Ala 215) was fused with a polyhistidine tag at the C-terminus, constructed the plasmid 1; A DNA sequence encoding the p40 subunit of mouse IL12, termed as IL12B (P43432) (Met 1-Ser 335) was fused with a polyhistidine tag at the C-terminus, constructed the plasmid 2. The two plasmids were co-expressed and the heterodimer was purified.
Description: Interleukin-12 (IL-12), also known as NKSF, TCMF, CLMF and TSF, is a heterodimeric cytokine composed of p35 and p40 subunits. It is produced by monocytes, macrophages, B cells and dendritic cells in response to bacterial lipopolysaccharides and intracellular pathogens. IL-12 signals through the IL-12 receptor complex, which is comprised of IL-12 Rβ1 and IL-12 Rβ2. IL-12 induces the proliferation and activation of hematopoietic stem cells, natural killer cells and T- cells. It is indispensible during the development of Th1 cells, leading to the production of IFN-gamma and IL-2.
Source: HEK293
Species: Mouse
Tag : His
Form: Lyophilized from sterile PBS, pH 7.4.
Bio-activity: 1. Measured by its ability to bind biotinylated recombinant mouse IL12RB2 in a functional ELISA.
2. Measured in a cell proliferation assay using Anti-CD3-stimulated PBMC. The ED50 for this effect is typically 2-8 ng/mL.
Molecular Mass: The recombinant mouse IL12 heterodimer of IL12A/IL12B, comprises 528 (204 + 324) amino acids and has a calculated molecular mass of 60.3 (23.1 + 37.2) kDa. The apparent molecular mass of rh IL12 heterodimer is approximately 28 & 47 kDa respectively in SDS-PAGE under reducing conditions.
Endotoxin: < 1.0 EU per μg of the protein as determined by the LAL method
Purity: > 90 % as determined by SDS-PAGE
Stability: Samples are stable for up to twelve months from date of receipt at -70 centigrade.
Storage: Store it under sterile conditions at -20 centigrade to -80 centigrade. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
Gene Name: interleukin 12a [ Mus musculus (house mouse) ]
Official Symbol: Il12a
Synonyms: CLMF; CLMF2; IL-12A; IL-12B; IL12; IMD28; IMD29; NFSK; NKSF; NKSF1;
NKSF2; P35; Il-12a; Il-12b; IL-12p35; Il-12p40; Il12p40; Ll12a; p35; p40
Gene ID: 16159
mRNA Refseq: NM_001159424.2
Protein Refseq: NP_001152896.1
UniProt ID: P43431

Online Inquiry

Note: There will be extra charge for optional service!
Optional requirements on this protein    +Expand
C-fusion    N-fusion   Non-tagged
His    GST   Fc   Others
<1.0 eu/μg    <0.1 eu/μg   <0.01 eu/μg   Not required
Monomer Isolation    Dimer Isolation    Not required
>80% by SDS-PAGE    >90% by SDS-PAGE   >95% by SDS-PAGE   Others

Please input "biomart" as verification code. Please review Creative BioMart's privacy policy for more information

Price does not include shipping and packaging costs.Request quote for final price.

Price Inquiry

Welcome! For price inquiries, please feel free to contact us through the form below. We will get back to you as soon as possible.