"IL12A/IL12B" Related Products

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Recombinant Mouse IL12A & IL12B Protein, Fc & His-tagged

Cat.No.: IL12A & IL12B-1244M
Product Overview: Recombinant p35 subunit of mouse IL12, termed as IL12A (P43431) (Met 1-Ala 215) was fused with a polyhistidine tag at the C-terminus, constructed the plasmid 1; Recombinant p40 subunit of mouse IL12, termed as IL12B (P43432) (Met 1-Ser 335) was fused with
Source: HEK293
Species: Mouse
Tag: Fc & His
Predicted N Terminal: Arg 23 & Met 23
Form: Lyophilized from sterile PBS, pH 7.4, 5%~8% trehalose and mannitol.
Bio-activity: 1. Measured by its ability to mouse IL12RB2-His in a functional ELISA. 2. Measured in a cell proliferation assay using Anti-CD3-stimulated PBMC. The ED50 for this effect is typically 0.2-1 ng/mL.
Molecular Mass: The recombinant mouse IL12 heterodimer of IL12A/IL12B, IL12B is Fc chimera, comprises 758 (204 + 554) amino acids and has a calculated molecular mass of 85.9 (23.1 + 62.8) kDa. The apparent molecular mass of rh IL12 heterodimer is approximately 28 & 75 kDa respectively in SDS-PAGE under reducing conditions.
Endotoxin: < 1.0 EU per μg of the protein as determined by the LAL method.
Purity: >85 % as determined by SDS-PAGE.
Stability: Samples are stable for up to twelve months from date of receipt at -70ºC.
Storage: Store it under sterile conditions at -20ºC~-70ºC. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
Reconstitution: It is recommended that sterile water be added to the vial to prepare a stock solution of 0.25 ug/ul. Centrifuge the vial at 4ºC before opening to recover the entire contents.
Gene Name: Il12a interleukin 12a [ Mus musculus ]
Official Symbol: IL12A
Synonyms: IL12A; interleukin 12a; interleukin-12 subunit alpha; CLMF p35; IL-12 subunit p35; interleukin 12a p35 subunit; cytotoxic lymphocyte maturation factor 35 kDa subunit; p35; Ll12a; Il-12a; IL-12p35; MGC151228; MGC151232;
Gene ID: 16159
mRNA Refseq: NM_001159424
Protein Refseq: NP_001152896
MIM:
UniProt ID:
Pathway: African trypanosomiasis, organism-specific biosystem; African trypanosomiasis, conserved biosystem; Allograft rejection, organism-specific biosystem; Allograft rejection, conserved biosystem; Amoebiasis, organism-specific biosystem; Amoebiasis, conserved
Function: contributes_to cytokine activity; cytokine activity; growth factor activity; interleukin-12 beta subunit binding; interleukin-12 receptor binding; interleukin-27 binding; protein heterodimerization activity;

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Optional requirements on this protein    +Expand
C-fusion    N-fusion   Non-tagged
His    GST   Fc   Others
<1.0 eu/μg    <0.1 eu/μg   <0.01 eu/μg   Not required
Monomer Isolation    Dimer Isolation    Not required
>80% by SDS-PAGE    >90% by SDS-PAGE   >95% by SDS-PAGE   Others

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