Recombinant Mouse Il12b protein(Met1-Ser335), hFc-tagged
Cat.No. : | Il12b-738M |
Product Overview : | Recombinant Mouse IL12B (P43432) (Met 1-Ser 335) was expressed in HEK293, fused with the Fc region of Human IgG1 at the C-terminus. |
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Source : | HEK293 |
Species : | Mouse |
Tag : | C-hFc |
Protein length : | Met1-Ser335 |
Form : | Lyophilized from sterile PBS, pH 7.4. Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization. |
Bio-activity : | 1. Measured by its binding ability in a functional ELISA. 2. Immobilized mouse IL12B-Fc at 10μg/mL (100μL/well) can bind biotinylated human IL12RB1-His. The EC50 of biotinylated human IL12RB1-His is 0.10-0.24μg/mL. |
Molecular Mass : | The secreted recombinant mouse IL12B/Fc is a disulfide-linked homodimer. The reduced monomer comprises 554 amino acids and has a calculated molecular mass of 62.8 kDa. As a result of glycosylation, the apparent molecular mass of the monomer is approximately 83 kDa in SDS-PAGE under reducing conditions. |
Endotoxin : | < 1.0 EU per μg of the protein as determined by the LAL method |
Purity : | > 90 % as determined by SDS-PAGE |
Storage : | Samples are stable for up to twelve months from date of receipt at -20°C to -80°C. Store it under sterile conditions at -20°C to -80°C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles. |
Reconstitution : | It is recommended that sterile water be added to the vial to prepare a stock solution of 0.2 ug/ul. Centrifuge the vial at 4°C before opening to recover the entire contents. |
Gene Name : | Il12b interleukin 12b [ Mus musculus ] |
Official Symbol : | Il12b |
Synonyms : | IL12B; interleukin 12b; interleukin-12 subunit beta; CLMF p40; IL-12 p40; IL-12 subunit p40; IL-23 subunit p40; cytotoxic lymphocyte maturation factor 40 kDa subunit; p40; Il-12b; Il12p40; Il-12p40; |
Gene ID : | 16160 |
mRNA Refseq : | NM_008352 |
Protein Refseq : | NP_032378 |
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For Research Use Only. Not intended for any clinical use. No products from Creative BioMart may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative BioMart.
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Q&As (10)
Ask a questionChIP-seq identifies regulatory elements controlling IL12B gene expression.
Reporter assays monitor IL12B promoter activity under varying conditions.
Primary cell stimulation assays gauge IL12B response to pathogens.
siRNA knockdown uncovers IL12B's role in immune cell activation pathways.
Recombinant protein expression enables functional IL12B studies in vitro.
Co-IP validates IL12B's binding to specific signaling partners.
Genetic variant analysis reveals impacts on IL12B protein function.
Flow cytometry profiles IL12B expression across diverse immune cell types.
Cytokine secretion assays measure IL12B's response kinetics to stimuli.
Co-IP and immunoprecipitation assess IL12B's interactions with other proteins.
Customer Reviews (3)
Write a reviewGood for cytokine assays.
Effective in Th2 cell differentiation.
High yield in expression.
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