Recombinant Mouse Il15 protein, Fc-tagged
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|Product Overview :||Recombinant Mouse Il15 (Asn49-Ser162) protein was fused to human IgG1 Fc tag at C-terminus and expressed in human 293 cells (HEK293).|
|Description :||Interleukin 15 is also known as IL15, IL-15, and is a cytokine with structural similarity to IL-2. Like IL-2, IL-15 binds to and signals through the IL-2/IL-15 beta chain (CD122) and the common gamma chain (gamma-C, CD132). IL-15 is secreted by mononuclear phagocytes (and some other cells) following infection by virus(es). This cytokine induces cell proliferation of natural killer cells; cells of the innate immune system whose principal role is to kill virally infected cells. Interleukin 15 (IL-15) regulates T and natural killer (NK) cell activation and proliferation. Survival signals that maintain memory T cells in the absence of antigen are provided by IL-15. This cytokine is also implicated in NK cell development. In rodent lymphocytes, IL-15 prevents apoptosis by inducing an apoptosis inhibitor, BCL2L1/BCL-x(L). IL-15 has been shown to enhance the anti-tumor immunity of CD8+ T cells in pre-clinical models. A phase I clinical trial to evaluate the safety, dosing, and anti-tumor efficacy of IL-15 in patients with metastatic melanoma and renal cell carcinoma (kidney cancer) has begun to enroll patients at the National Institutes of Health.|
|Tag :||human IgG1 Fc|
|Predicted N Terminal :||Asn49|
|Form :||Lyophilized from 0.22 μm filtered solution in Tris with Glycine, Arginine and NaCl, pH7.5, 10% trehalose.|
|Molecular Mass :||The protein has a calculated MW of 39.7 kDa. The protein migrates as 49 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.|
|Protein length :||Asn49-Ser162|
|Endotoxin :||Less than 1.0 EU per μg by the LAL method.|
|Purity :||>95% as determined by SDS-PAGE.|
For long term storage, the product should be stored at lyophilized state at -20 centigrade or lower.
Please avoid repeated freeze-thaw cycles.
This product is stable after storage at:
-20 to -70 centigrade for 12 months in lyophilized state;
-70 centigrade for 3 months under sterile conditions after reconstitution.
|Reconstitution :||It is recommended that sterile water be added to the vial to prepare a stock solution of 0.2 ug/ul. Centrifuge the vial at 4ºC before opening to recover the entire contents.|
|SDS-PAGE of Il15-647M :||
Mouse IL-15, Fc Tag on SDS-PAGE under reducing (R) condition. The gel was stained overnight with Coomassie Blue. The purity of the protein is greater than 95%.
|Bioactivity-ELISA of Il15-647M :||
Immobilized Mouse IL-15, Fc Tag at 2 μg/mL (100 μL/well) can bind Biotinylated Human IL-15 R alpha, Fc,Avitag with a linear range of 10-156 ng/mL (QC tested).
|Gene Name :||Il15|
|Official Symbol :||Il15|
|Synonyms :||IL15; interleukin 15; interleukin-15; IL-15; AI503618|
|Gene ID :||16168|
|mRNA Refseq :||NM_001254747|
|Protein Refseq :||NP_001241676|
|UniProt ID :||P48346|
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