Recombinant Mouse Il17d Protein, His-tagged

• Cat.No.: Il17d-1664M
• Product Overview: Recombinant Mouse Il17d Protein (Ala23-Arg205) with N-His tag was expressed in E. coli.

Specification

• Description: Predicted to enable cytokine activity and protein homodimerization activity. Predicted to be involved in inflammatory response; negative regulation of hemopoiesis; and positive regulation of cytokine production. Predicted to be located in extracellular space. Is expressed in central nervous system; ganglia; nucleus pulposus; and thymus primordium. Orthologous to human IL17D (interleukin 17D).
• Source: E. coli
• Species: Mouse
• Tag: His
• Form: Freeze-dried powder
• Molecular Mass: Predicted Molecular Mass: 23.8 kDa
  Accurate Molecular Mass: 24 kDa
• Protein length: Ala23-Arg205
• Purity: > 95%
• Applications: Positive Control; Immunogen; SDS-PAGE; WB.
• Stability: The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37 centigrade for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.
• Storage: Avoid repeated freeze/thaw cycles. Store at 2-8 centigrade for one month. Aliquot and store at -80 centigrade for 12 months.
• Storage Buffer: 20mM Tris, 150mM NaCl, pH8.0, containing 1mM EDTA, 1mM DTT, 0.01% SKL, 5% Trehalose and Proclin300.
• Reconstitution: Reconstitute in 20mM Tris, 150mM NaCl (pH8.0) to a concentration of 0.1-1.0 mg/mL. Do not vortex.

Gene Information

• Gene Name: Il17d interleukin 17D [ Mus musculus (house mouse) ]
• Official Symbol: Il17d
• Synonyms: IL17D; interleukin 17D; interleukin 27A; Il27a; IL-17D; AI462269
• Gene ID: 239114
• mRNA Refseq: NM_145837
• Protein Refseq: NP_665836
• UniProt ID: Q8K4C4

Reviews

12/03/2022

Effective in Th9 differentiation.

08/14/2019

Suitable for ELISA.

08/12/2019

High bioactivity.

Q&As

What methodologies are employed to explore the role of IL17D in neuroinflammation and its potential implications in neurological disorders?

Neuroinflammation models, neuronal cell assays, and CNS analysis explore IL17D's role in neurological contexts.

What techniques are used to characterize the potential pro-tumor or anti-tumor effects of IL17D in cancer development and progression?

Tumor xenografts, immune cell infiltration analysis, and gene expression profiling dissect IL17D's role in cancer.

How do researchers decipher the distinct roles of IL17D compared to other IL17 family members in immune responses?

Functional assays, knockout models, and gene expression analyses distinguish IL17D's unique roles.

How is the interaction between IL17D and the gut microbiota investigated, and what are the implications for intestinal homeostasis?

Microbiota sequencing, gnotobiotic models, and immune cell profiling investigate IL17D-gut microbiota interactions.

How are the effects of IL17D modulation on autoimmune diseases investigated using experimental models and patient samples?

Animal models, patient samples, and genetic studies elucidate IL17D's involvement in autoimmune diseases.

Can you explain the experimental designs used to study the involvement of IL17D in wound healing and tissue regeneration?

Wound healing assays, tissue regeneration studies, and histological analyses examine IL17D's effects on tissue repair.

What experimental techniques are employed to investigate the signaling pathways triggered by IL17D and its potential crosstalk with other cytokines?

Signal transduction assays, phosphoproteomics, and cytokine profiling reveal IL17D's signaling pathways.

What molecular approaches are utilized to assess IL17D's impact on host defense against infections and regulation of mucosal immunity?

Cell culture models, infection assays, and epithelial barrier assessments uncover IL17D's impact on host defense.

Could you elaborate on the strategies used to study IL17D's contribution to tissue-specific immune cell activation and recruitment?

Flow cytometry, chemotaxis assays, and adoptive transfer experiments probe IL17D-mediated immune responses.

How are the therapeutic applications of IL17D modulation studied, and what approaches are taken to validate its potential as a therapeutic target?

Therapeutic antibody testing, RNA interference, and animal disease models validate IL17D as a potential therapy.