Active Recombinant Mouse IL3 protein, His-tagged

Cat.No. : IL3-88M
Product Overview : Active Recombinant Mouse IL3 protein(P01586)(33~166 aa), fused with N-terminal His tag, was expressed in HEK293.
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Species : Mouse
Source : HEK293
Tag : His
Protein Length : 33-166 aa
Form : Lyophilized from sterile PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
Bio-activity : Interleukin 3 is an interleukin, a type of biological signal that can improve the body's natural response to disease as part of the immune system. It acts by binding to the interleukin 3 receptor IL-3 synergizes with other cytokines to stimulate the growth of immature progenitor cells of all lineages, and is therefore a multi-lineage colony-stimulating factor (CSF). It prevents cell death and promotes the survival of macrophages, mast cells, and megakaryocytes. To test the effect of IL-3 on cell proliferation, TF-1 cells were seeded into triplicate wells of 96-well plates at a density of 5,000 cells/well with 2% serum standard 1640 which contains various concentrations of recombinant mouse IL-3. After incubated for 72h, cells were observed by inverted microscope and cell proliferation was measured by Cell Counting Kit-8 (CCK-8). Briefly, 10 µl of CCK-8 solution was added to each well of the plate, then the absorbance at 450 nm was measured using a microplate reader after incubating the plate for 2-4 hours at 37°C. Proliferation of TF-1 cells after incubation with IL-3 for 72h observed by inverted microscope was shown. Cell viability was assessed by CCK-8(Cell Counting Kit-8 ) assay after incubation with recombinant IL-3 for 72h. It was obvious that IL-3 significantly increased cell viability of TF-1 cells. The ED50 is 3.79 ng/ml.
Molecular Mass : 16.7kDa
Endotoxin : <1.0EU per 1μg (determined by the LAL method).
Purity : > 95% as determined by SDS-PAGE.
Storage : Avoid repeated freeze/thaw cycles. Store at -20°C for 12 months. Aliquot and store at -80°C for 12 months.
Reconstitution : Reconstitute in 10mM PBS (pH7.4) to a concentration of 0.1-1.0 mg/mL. Do not vortex.
Gene Name Il3 interleukin 3 [ Mus musculus ]
Official Symbol IL3
Synonyms IL3; interleukin 3; interleukin-3; mast cell growth factor; P-cell-stimulating factor; hematopoietic growth factor; multipotential colony-stimulating factor; BPA; PSF; HCGF; Il-3; MCGF; Csfmu;
Gene ID 16187
mRNA Refseq NM_010556
Protein Refseq NP_034686

Not For Human Consumption!

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