Active Recombinant Mouse Il33 protein

• Cat.No.: Il33-207M
• Product Overview: Recombinant Mouse Il33 protein(Ser109-Ile266) was expressed in E.coli.

Specification

• Species: Mouse
• Source: E.coli
• Tag: Non
• Protein Length: 109-266 aa
• Form: Lyophilized from a 0.2 μM filtered solution of 20mM PB,150mM NaCl,pH7.4
• Bio-activity: Measured in a cell proliferation assay using D10.G4.1 mouse helper T cells. The ED50 for this effect is 0.0745-0.298 ng/mL, corresponding to a specific activity of 3.36×10^6~1.34×10^7 units/mg.
• Molecular Mass: 17.6 kDa
• Endotoxin: < 1 EU/μg of the protein by LAL method.
• Purity: ≥ 95 % as determined by SDS-PAGE.
• Storage: Store at -20°C.Store the lyophilized protein at -20°C to -80°C up to 1 year from the date of receipt.After reconstitution, the protein solution is stable at -20°C for 3 months, at 2-8°C for up to 1 week.
• Reconstitution: Centrifuge the vial before opening. Reconstitute to a concentration of 0.1-0.5 mg/mL in sterile distilled water. Avoid vortex or vigorously pipetting the protein. For long term storage, it is recommended to add a carrier protein or stablizer (e.g. 0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose), and aliquot the reconstituted protein solution to minimize free-thaw cycles.

Gene Information

• Gene Name: Il33 interleukin 33 [ Mus musculus ]
• Official Symbol: Il33
• Synonyms: IL33; interleukin 33; interleukin-33; nuclear factor from high endothelial venules; Il-33; Il1f11; NF-HEV; 9230117N10Rik
• Gene ID: 77125
• mRNA Refseq: NM_001164724
• Protein Refseq: NP_001158196