| Cat. No. : |
Il20-74M |
| Description : |
IL20 is a cytokine structurally related to interleukin 10 (IL10). IL20 cytokine has been shown to transduce its signal through signal transducer and activator of transcription 3 (STAT3) in keratinocytes. A specific receptor for this cytokine is found to be expressed in skin and upregulated dramatically in psoriatic skin, suggesting a role for this protein in epidermal function and psoriasis. |
| Source : |
Escherichia Coli. |
| Amino Acid Sequence : |
The sequence of the first five N-terminal amino acids was determined and was found to be Met-Leu-Lys-Thr-Leu. |
| Physical Appearance : |
Sterile Filtered White lyophilized (freeze-dried) powder. |
| Purity : |
Greater than 95.0% as determined by: (a) Analysis by RP-HPLC. (b) Analysis by SDS-PAGE. |
| Formulation : |
Lyophilized from a concentrated (1 mg/ml) solution containing no additives. |
| Solubility : |
It is recommended to reconstitute the lyophilized Interleukin-20 in sterile 18 MΩ-cm H2O not less than 100 µg/ml, which can then be further diluted to other aqueous solutions. |
| Specific Activity : |
The biological activity was determined by measuring the dose dependent activation of STAT3 in human epidermal keratinocytes (HEK2a cells). Significant STAT3 activation is observed with >100 ng/mL of Interleukin-20 recombinant mouse. |
| Protein Content : |
Protein quantitation was carried out by two independent methods: 1. UV spectroscopy at 280 nm using the absorbency value of 0.552 as the extinction coefficient for a 0.1% (1mg/ml) solution. This value is calculated by the PC GENE computer analysis program of protein sequences (IntelliGenetics). 2. Analysis by RP-HPLC, using a standard solution of IL-20 as a Reference Standard. |
| Stability : |
Lyophilized Interleukin-20 although stable at room temperature for 3 weeks, should be stored desiccated below -18°C. Upon reconstitution IL20 should be stored at 4°Cbetween 2-7 days and for future use below -18°C. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA). Please prevent freeze-thaw cycles. |
