| Cat. No. : |
Il4ra-1757M |
| Description : |
Interleukin 4 receptor is a type I cytokine receptor. IL4R is its human gene. This gene encodes the alpha chain of the interleukin-4 receptor, a type I transmembrane protein that can bind interleukin 4 and interleukin 13 to regulate IgE antibody production in B cells. Among T cells, the encoded protein also can bind interleukin 4 to promote differentiation of Th2 cells. A soluble form of the encoded protein can be produced by an alternate splice variant or by proteolysis of the membrane-bound protein, and this soluble form can inhibit IL4-mediated cell proliferation and IL5 upregulation by T-cells. |
| Molecular Weight : |
The predicted molecular weight of Recombinant Mouse IL-4 R is Mr 51 kDa. However, the actual molecular weight as observed by migration on SDS Page is Mr 65-80 kDa. |
| Source : |
Mammalian cells |
| State Of Matter : |
Lyophilized. |
| Purity : |
>90% by SDS Page and analyzed by silver stain. |
| Formulation : |
This recombinant protein was 0.2 µm filtered and lyophilized from modified Dulbecco's phosphate buffered saline (1X PBS) pH 7.2 – 7.3 with no calcium, magnesium, or preservatives. |
| Endotoxin : |
<0.1 ng per 1 µg as determined by the LAL method. |
| Biological Activity : |
The biological activity of Mouse IL-4 R was determined by its ability to inhibit the IL-4-dependent proliferation of HT2 cells. The expected ED50 for this effect is typically 0.05-0.2 μg/ml in the presence of 3 ng/ml of Mouse IL-4. |
| Storage And Stability : |
This lyophilized protein is stable for six to twelve months when stored desiccated at -20°Cto -70°C. After aseptic reconstitution, this protein may be stored at 2°Cto 8°Cfor one month or at -20°Cto -70°Cin a manual defrost freezer. Avoid Repeated Freeze Thaw Cycles. See Product Insert for exact lot specific storage instructions. |
