Recombinant Mouse Iscu Protein, Myc/DDK-tagged

• Cat.No.: Iscu-3583M
• Product Overview: Purified recombinant protein of mouse full-length iron-sulfur cluster assembly enzyme (Iscu), with C-terminal MYC/DDK tag, expressed in HEK293T cells.

Specification

• Species: Mouse
• Source: HEK293
• Tag: DDK&Myc
• Description: Scaffold protein for the de novo synthesis of iron-sulfur (Fe-S) clusters within mitochondria, which is required for maturation of both mitochondrial and cytoplasmic [2Fe-2S] and [4Fe-4S] proteins. First, a [2Fe-2S] cluster is transiently assembled on the scaffold protein ISCU. In a second step, the cluster is released from ISCU, transferred to a glutaredoxin GLRX5, followed by the formation of mitochondrial [2Fe-2S] proteins, the synthesis of [4Fe-4S] clusters and their target-specific insertion into the recipient apoproteins. Cluster assembly on ISCU depends on the function of the cysteine desulfurase complex NFS1-LYRM4/ISD11, which serves as the sulfur donor for cluster synthesis, the iron-binding protein frataxin as the putative iron donor, and the electron transfer chain comprised of ferredoxin reductase and ferredoxin, which receive their electrons from NADH.
• Molecular Mass: 18.1 kDa
• Purity: > 80% as determined by SDS-PAGE and Coomassie blue staining
• Stability: Stable for 12 months from the date of receipt of the product under proper storage and handling conditions. Avoid repeated freeze-thaw cycles.
• Storage: Store at -80 centigrade after receiving vials.
• Concentration: >50 μg/mL as determined by microplate BCA method
• Storage Buffer: 25 mM Tris.HCl, pH 7.3, 100 mM glycine, 10% glycerol.

Gene Information

• Gene Name: Iscu iron-sulfur cluster assembly enzyme [ Mus musculus (house mouse) ]
• Official Symbol: Iscu
• Synonyms: ISCU; IscU iron-sulfur cluster scaffold homolog (E. coli); iron-sulfur cluster assembly enzyme ISCU, mitochondrial; ISCU2; nifU-like protein; NifU-like N-terminal domain containing; nifU-like N-terminal domain-containing protein; Nifu; Nifun; AA407971; 2310020H20Rik
• Gene ID: 66383
• mRNA Refseq: NM_025526
• Protein Refseq: NP_079802
• UniProt ID: Q9D7P6