Recombinant Mouse KPNB1 Protein (1-876 aa), His-tagged

• Cat.No.: KPNB1-609M
• Product Overview: Recombinant Mouse KPNB1 Protein (1-876 aa) is produced by E. coli expression system. This protein is fused with a 6xHis tag at the N-terminal. Protein Description: Full Length.

Specification

• Description: Functions in nuclear protein import, either in association with an adapter protein, like an importin-alpha subunit, which binds to nuclear localization signals (NLS) in cargo substrates, or by acting as autonomous nuclear transport receptor. Acting autonomously, serves itself as NLS receptor. Docking of the importin/substrate complex to the nuclear pore complex (NPC) is mediated by KPNB1 through binding to nucleoporin FxFG repeats and the complex is subsequently translocated through the pore by an energy requiring, Ran-dependent mechanism. At the nucleoplasmic side of the NPC, Ran binds to importin-beta and the three components separate and importin-alpha and -beta are re-exported from the nucleus to the cytoplasm where GTP hydrolysis releases Ran from importin. The directionality of nuclear import is thought to be conferred by an asymmetric distribution of the GTP- and GDP-bound forms of Ran between the cytoplasm and nucleus. Mediates autonomously the nuclear import of ribosomal proteins RPL23A, RPS7 and RPL5. Binds to a beta-like import receptor binding (BIB) domain of RPL23A. In association with IPO7 mediates the nuclear import of H1 histone. In vitro, mediates nuclear import of H2A, H2B, H3 and H4 histones. In case of HIV-1 infection, binds and mediates the nuclear import of HIV-1 Rev. Imports SNAI1 and PRKCI into the nucleus.
• Source: E. coli
• Species: Mouse
• Tag: His
• Form: Tris-based buffer, 50% glycerol
• Molecular Mass: 101.2 kDa
• Protein length: 1-876 aa
• AA Sequence: MELITILEKTVSPDRLELEAAQKFL ERAAVENLPTFLVELSRVLANPGNS QVARVAAGLQIKNSLTSKDPDIKAQ YQQRWLAIDANARREVKNYVLQTLG TETYRPSSASQCVAGIACAEIPVSQ WPELIPQLVANVTNPNSTEHMKEST LEAIGYICQDIDPEQLQDKSNEILT AIIQGMRKEEPSNNVKLAATNALLN SLEFTKANFDKESERHFIMQVVCEA TQCPDTRVRVAALQNLVKIMSLYYQ YMETYMGPALFAITIEAMKSDIDEV ALQGIEFWSNVCDEEMDLAIEASEA AEQGRPPEHTSKFYAKGALQYLVPI LTQTLTKQDENDDDDDWNPCKAAGV CLMLLSTCCEDDIVPHVLPFIKEHI KNPDWRYRDAAVMAFGSILEGPEPN QLKPLVIQAMPTLIELMKDPSVVVR DTTAWTVGRICELLPEAAINDVYLA PLLQCLIEGLSAEPRVASNVCWAFS SLAEAAYEAADVADDQEEPATYCLS SSFELIVQKLLETTDRPDGHQNNLR SSAYESLMEIVKNSAKDCYPAVQKT TLVIMERLQQVLQMESHIQSTSDRI QFNDLQSLLCATLQNVLRKVQHQDA LQISDVVMASLLRMFQSTAGSGGVQ EDALMAVSTLVEVLGGEFLKYMEAF KPFLGIGLKNYAEYQVCLAAVGLVG DLCRALQSNILPFCDEVMQLLLENL GNENVHRSVKPQILSVFGDIALAIG GEFKKYLEVVLNTLQQASQAQVDKS DFDMVDYLNELRESCLEAYTGIVQG LKGDQENVHPDVMLVQPRVEFILSF IDHIAGDEDHTDGVVACAAGLIGDL CTAFGKDVLKLVEARPMIHELLTEG RRSKTNKAKTLATWATKELRKLKNQ A
• Purity: > 90% as determined by SDS-PAGE.
• Notes: Repeated freezing and thawing is not recommended. Store working aliquots at 4 centigrade for up to one week.
• Storage: The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself. Generally, the shelf life of liquid form is 6 months at -20 centigrade/-80 centigrade. The shelf life of lyophilized form is 12 months at -20 centigrade/-80 centigrade.
• Concentration: A hardcopy of COA with concentration instruction is sent along with the products.

Gene Information

• Gene Name: Kpnb1 karyopherin (importin) beta 1 [ Mus musculus ]
• Official Symbol: KPNB1
• Synonyms: KPNB1; SCG; PTAC97; IPOB; Impnb; AA409963; MGC8315
• Gene ID: 16211
• mRNA Refseq: NM_008379
• Protein Refseq: NP_032405
• UniProt ID: P70168