"Ldha" Related Products

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Recombinant Mouse Ldha Protein, His-tagged

Cat.No.: Ldha-61M
Product Overview: Recombinant mouse Ldha, fused to His-tag at C-terminus, was expressed in insect cell and purified by using conventional chromatography techniques.
Description: Ldha, also known as L-lactate dehydrogenase A chain isoform 1, is a member of the LDH/MDH superfamily and LDH family. It catalyzes the conversion of L-lactate and NAD to pyruvate and NADH in the final step of anaerobic glycolysis. This protein is found predominantly in muscle tissue. It has long been known that many human cancers have higher LDHA levels compared to normal tissues. It plays an important role in the development, invasion and metastasis of malignancies.
Source: Insect cells
Species: Mouse
Tag: His tag
Form: Liquid. In Phosphate Buffered Saline (pH 7.4) containing 10% glycerol.
Bio-activity: Specific activity is >250 units/mg, and is defined as the Amount of enzyme that convert 1.0 umole of pyruvate to L-lactate and per minute at pH 7.5 at 37 centigrade.
Molecular Mass: 37.5kDa (340aa)28-40KDa (SDS–PAGE under reducing conditions.)
AA Sequence: MATLKDQLIV NLLKEEQAPQ NKITVVGVGA VGMACAISIL MKDLADELAL VDVMEDKLKG EMMDLQHGSL FLKTPKIVSS KDYCVTANSKLVIITAGARQ QEGESRLNLV QRNVNIFKFI IPNIVKYSPH CKLLIVSNPV DILTYVAWKI SGFPKNRVIG SGCNLDSARF RYLMGERLGVHALSCHGWVL GEHGDSSVPV WSGVNVAGVS LKSLNPELGT DADKEQWKEV HKQVVDSAYE VIKLKGYTSW AIGLSVADLA ESIMKNLRRVHPISTMIKGL YGINEDVFLS VPCILGQNGI SDVVKVTLTP EEEARLKKSA DTLWGIQKEL QFLEHHHHHH
Endotoxin: < 1.0 EU per 1 microgram of protein (determined by LAL method)
Purity: >95% by SDS - PAGE
Storage: Can be stored at +4centigrade short term (1-2 weeks). For long term storage, aliquot and store at -20Centigrade or -70Centigrade. Avoid repeated freezing and thawing cycles.
Concentration: 0.5mg/ml (determined by Absorbance at 280nm)
Gene Name: Ldha lactate dehydrogenase A [ Mus musculus ]
Official Symbol: Ldha
Synonyms: LDHA; LDH-M; M-LDH; Ldh1; Ldhm; l7R2;
Gene ID: 16828
mRNA Refseq: NM_001136069
Protein Refseq: NP_001129541
MIM:
UniProt ID:
Pathway: Cysteine and methionine metabolism, organism-specific biosystem; Cysteine and methionine metabolism, conserved biosystem; Glycolysis / Gluconeogenesis, organism-specific biosystem; Glycolysis / Gluconeogenesis, conserved biosystem; Glycolysis and Gluconeogenesis, organism-specific biosystem; Metabolic pathways, organism-specific biosystem; Metabolism, organism-specific biosystem;
Function: L-lactate dehydrogenase activity; L-lactate dehydrogenase activity; L-lactate dehydrogenase activity; NAD binding; catalytic activity; identical protein binding; kinase binding; lactate dehydrogenase activity; nucleotide binding

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Optional requirements on this protein    +Expand
C-fusion    N-fusion   Non-tagged
His    GST   Fc   Others
<1.0 eu/μg    <0.1 eu/μg   <0.01 eu/μg   Not required
Monomer Isolation    Dimer Isolation    Not required
>80% by SDS-PAGE    >90% by SDS-PAGE   >95% by SDS-PAGE   Others

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