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Active Recombinant Mouse LIFR protein(Met1-Ser828), His-tagged

Cat.No. : LIFR-868M
Product Overview : Recombinant Mouse LIFR (NP_038612.1) (Met 1-Ser 828) was expressed in HEK293, with a polyhistidine tag at the C-terminus.
  • Specification
  • Gene Information
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Source : HEK293
Species : Mouse
Tag : C-His
Protein length : Met1-Ser828
Form : Lyophilized from sterile PBS, pH 7.4. Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization.
Bio-activity : 1. Measured by its ability to bind mouse LIF-Fc in a functional ELISA. 2. Measured by its ability to inhibit the recombinant human LIF mediated inhibition in the M1 mouse myeloid leukemia cells. The ED50 for this effect is typically 5-20 ng/mL in the presence of 2 ng/mL recombinant human LIF.
Molecular Mass : The recombinant mouse LIFR consists of 796 amino acids and has a predicted molecular mass of 90 kDa. In SDS-PAGE under reducing conditions, the apparent molecular mass of rm LIFR is approximately 110-120 kDa due to glycosylation.
Endotoxin : < 1.0 EU per μg of the protein as determined by the LAL method.
Purity : > 95 % as determined by SDS-PAGE
Storage : Samples are stable for up to twelve months from date of receipt at -20°C to -80°C. Store it under sterile conditions at -20°C to -80°C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
Reconstitution : It is recommended that sterile water be added to the vial to prepare a stock solution of 0.2 ug/ul. Centrifuge the vial at 4°C before opening to recover the entire contents.
Gene Name : Lifr leukemia inhibitory factor receptor [ Mus musculus ]
Official Symbol : LIFR
Synonyms : LIFR; leukemia inhibitory factor receptor; LIF-R; LIF receptor; D-factor/LIF receptor; leukemia inhibitory factor receptor alpha chain; soluble differentiation-stimulating factor receptor; LIF; AW061234; A230075M04Rik;
Gene ID : 16880
mRNA Refseq : NM_001113386
Protein Refseq : NP_001106857

For Research Use Only. Not intended for any clinical use. No products from Creative BioMart may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative BioMart.

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