Recombinant Mouse Mad2l1 Protein, Myc/DDK-tagged

• Cat.No.: Mad2l1-3895M
• Product Overview: Purified recombinant protein of mouse full-length MAD2 mitotic arrest deficient-like 1 (Mad2l1), with C-terminal MYC/DDK tag, expressed in HEK293T cells.

Specification

• Description: Component of the spindle-assembly checkpoint that prevents the onset of anaphase until all chromosomes are properly aligned at the metaphase plate. In the closed conformation (C-MAD2) forms a heterotetrameric complex with MAD1L1 at unattached kinetochores during prometaphase, and recruits an open conformation of MAD2L1 (O-MAD2) which then promotes the conversion of O-MAD2 to C-MAD2. Required for the execution of the mitotic checkpoint which monitors the process of kinetochore-spindle attachment and inhibits the activity of the anaphase promoting complex by sequestering CDC20 until all chromosomes are aligned at the metaphase plate.
• Source: HEK293T
• Species: Mouse
• Tag: Myc/DDK
• Molecular Mass: 24 kDa
• Purity: > 80% as determined by SDS-PAGE and Coomassie blue staining
• Stability: Stable for 12 months from the date of receipt of the product under proper storage and handling conditions. Avoid repeated freeze-thaw cycles.
• Storage: Store at -80 centigrade after receiving vials.
• Concentration: >50 μg/mL as determined by microplate BCA method
• Storage Buffer: 25 mM Tris.HCl, pH 7.3, 100 mM glycine, 10% glycerol.

Gene Information

• Gene Name: Mad2l1 MAD2 mitotic arrest deficient-like 1 [ Mus musculus (house mouse) ]
• Official Symbol: Mad2l1
• Synonyms: MAD2L1; MAD2 mitotic arrest deficient-like 1 (yeast); mitotic spindle assembly checkpoint protein MAD2A; MAD2-like protein 1; mitotic arrest deficient 2-like protein 1; MAD2 (mitotic arrest deficient, homolog)-like 1; MAD2; AA673185; MGC113763
• Gene ID: 56150
• mRNA Refseq: NM_019499
• Protein Refseq: NP_062372
• UniProt ID: Q9Z1B5