Recombinant Mouse Map2k7 Protein, Myc/DDK-tagged

• Cat.No.: Map2k7-3925M
• Product Overview: Purified recombinant protein of mouse full-length mitogen-activated protein kinase kinase 7 (Map2k7), with C-terminal MYC/DDK tag, expressed in HEK293T cells.

Specification

• Species: Mouse
• Source: HEK293
• Tag: DDK&Myc
• Description: Dual specificity protein kinase which acts as an essential component of the MAP kinase signal transduction pathway. Essential component of the stress-activated protein kinase/c-Jun N-terminal kinase (SAP/JNK) signaling pathway. With MAP2K4/MKK4, is the one of the only known kinase to directly activate the stress-activated protein kinase/c-Jun N-terminal kinases MAPK8/JNK1, MAPK9/JNK2 and MAPK10/JNK3. MAP2K4/MKK4 and MAP2K7/MKK7 both activate the JNKs by phosphorylation, but they differ in their preference for the phosphorylation site in the Thr-Pro-Tyr motif. MAP2K4/MKK4 shows preference for phosphorylation of the Tyr residue and MAP2K7/MKK7 for the Thr residue. The monophosphorylation of JNKs on the Thr residue is sufficient to increase JNK activity indicating that MAP2K7/MKK7 is important to trigger JNK activity, while the additional phosphorylation of the Tyr residue by MAP2K4/MKK4 ensures optimal JNK activation. Has a specific role in JNK signal transduction pathway activated by proinflammatory cytokines. The MKK/JNK signaling pathway is also involved in mitochondrial death signaling pathway, including the release cytochrome c, leading to apoptosis. Part of a non-canonical MAPK signaling pathway, composed of the upstream MAP3K12 kinase and downstream MAP kinases MAPK1/ERK2 and MAPK3/ERK1, that enhances the AP-1-mediated transcription of APP in response to APOE.
• Molecular Mass: 52.9 kDa
• Purity: > 80% as determined by SDS-PAGE and Coomassie blue staining
• Stability: Stable for 12 months from the date of receipt of the product under proper storage and handling conditions. Avoid repeated freeze-thaw cycles.
• Storage: Store at -80 centigrade after receiving vials.
• Concentration: >50 μg/mL as determined by microplate BCA method
• Storage Buffer: 25 mM Tris.HCl, pH 7.3, 100 mM glycine, 10% glycerol.

Gene Information

• Gene Name: Map2k7 mitogen-activated protein kinase kinase 7 [ Mus musculus (house mouse) ]
• Official Symbol: Map2k7
• Synonyms: MAP2K7; mitogen-activated protein kinase kinase 7; dual specificity mitogen-activated protein kinase kinase 7; MEK 7; JNKK 2; MAPKK 7; JNK kinase 2; MAPK/ERK kinase 7; MAP kinase kinase 7; JNK-activating kinase 2; c-Jun N-terminal kinase kinase 2; mitogen activated protein kinase kinase 7; protein kinase, mitogen activated, kinase 7; MKK7; sek2; Jnkk2; Prkmk7; 5930412N11Rik
• Gene ID: 26400
• mRNA Refseq: NM_001042557
• Protein Refseq: NP_001036022
• UniProt ID: Q8CE90