Recombinant Mouse Mapkap1 Protein, Myc/DDK-tagged

• Cat.No.: Mapkap1-3949M
• Product Overview: Purified recombinant protein of mouse full-length mitogen-activated protein kinase associated protein 1 (Mapkap1), with C-terminal MYC/DDK tag, expressed in HEK293T cells.

Specification

• Description: Subunit of mTORC2, which regulates cell growth and survival in response to hormonal signals. mTORC2 is activated by growth factors, but, in contrast to mTORC1, seems to be nutrient-insensitive. mTORC2 seems to function upstream of Rho GTPases to regulate the actin cytoskeleton, probably by activating one or more Rho-type guanine nucleotide exchange factors. mTORC2 promotes the serum-induced formation of stress-fibers or F-actin. mTORC2 plays a critical role in AKT1 'Ser-473' phosphorylation, which may facilitate the phosphorylation of the activation loop of AKT1 on 'Thr-308' by PDK1 which is a prerequisite for full activation. mTORC2 regulates the phosphorylation of SGK1 at 'Ser-422'. mTORC2 also modulates the phosphorylation of PRKCA on 'Ser-657'. Within mTORC2, MAPKAP1 is required for complex formation and mTORC2 kinase activity. MAPKAP1 inhibits MAP3K2 by preventing its dimerization and autophosphorylation. Inhibits HRAS and KRAS signaling. Enhances osmotic stress-induced phosphorylation of ATF2 and ATF2-mediated transcription. Isoform 1 is involved in ciliogenesis, regulates cilia length through its interaction with CCDC28B independently of mTORC2 complex.
• Source: HEK293T
• Species: Mouse
• Tag: Myc/DDK
• Molecular Mass: 59.5 kDa
• Purity: > 80% as determined by SDS-PAGE and Coomassie blue staining
• Stability: Stable for 12 months from the date of receipt of the product under proper storage and handling conditions. Avoid repeated freeze-thaw cycles.
• Storage: Store at -80 centigrade after receiving vials.
• Concentration: >50 μg/mL as determined by microplate BCA method
• Storage Buffer: 25 mM Tris.HCl, pH 7.3, 100 mM glycine, 10% glycerol.

Gene Information

• Gene Name: Mapkap1 mitogen-activated protein kinase associated protein 1 [ Mus musculus (house mouse) ]
• Official Symbol: Mapkap1
• Synonyms: MAPKAP1; mitogen-activated protein kinase associated protein 1; target of rapamycin complex 2 subunit MAPKAP1; TORC2 subunit MAPKAP1; SAPK-interacting protein 1; stress-activated map kinase-interacting protein 1; mitogen activated protein kinase associated protein 1; mitogen-activated protein kinase 2-associated protein 1; Sin1; AI591529; D230039K05Rik
• Gene ID: 227743
• mRNA Refseq: NM_177345
• Protein Refseq: NP_796319
• UniProt ID: Q8BKH7