Recombinant Mouse Marchf8 Protein, Myc/DDK-tagged

• Cat.No.: Marchf8-3958M
• Product Overview: Purified recombinant protein of mouse full-length membrane-associated ring finger (C3HC4) 8 (March8), with C-terminal MYC/DDK tag, expressed in HEK293T cells.

Specification

• Species: Mouse
• Source: HEK293
• Tag: DDK&Myc
• Description: The protein encoded by this gene is a member of the membrane-associated really interesting new gene-CH family of proteins. These proteins are E3 ubiquitin-protein ligases that modulate antigen presentation by downregulating major histocompatibility complex class II surface expression through endocytosis. The transcript is primarily expressed by dendritic cells and macrophages. Overexpression of this gene in antigen presenting cells results in immune defective phenotypes, including resistance to autoimmune disease onset. Alternative splicing results in multiple transcript variants.
• Molecular Mass: 32.2 kDa
• Purity: > 80% as determined by SDS-PAGE and Coomassie blue staining
• Stability: Stable for 12 months from the date of receipt of the product under proper storage and handling conditions. Avoid repeated freeze-thaw cycles.
• Storage: Store at -80 centigrade after receiving vials.
• Concentration: >50 μg/mL as determined by microplate BCA method
• Storage Buffer: 25 mM Tris.HCl, pH 7.3, 100 mM glycine, 10% glycerol.

Gene Information

• Gene Name: Marchf8 membrane associated ring-CH-type finger 8 [ Mus musculus (house mouse) ]
• Official Symbol: Marchf8
• Synonyms: Marchf8; membrane associated ring-CH-type finger 8; M; Mir; Marc; March8; MARCH-VIII; 1300017E09Rik; E3 ubiquitin-protein ligase MARCHF8; E3 ubiquitin-protein ligase MARCH8; RING-type E3 ubiquitin transferase MARCH8; RING-type E3 ubiquitin transferase MARCHF8; c-mir, cellular modulator of immune recognition; cellular modulator of immune recognition; membrane-associated RING finger protein 8; membrane-associated RING-CH protein VIII; membrane-associated ring finger (C3HC4) 8; EC 2.3.2.27
• Gene ID: 71779
• mRNA Refseq: NM_027920
• Protein Refseq: NP_082196
• UniProt ID: Q9DBD2