Recombinant Mouse Mcrip1 Protein, Myc/DDK-tagged

• Cat.No.: Mcrip1-3999M
• Product Overview: Purified recombinant protein of mouse full-length MAPK regulated corepressor interacting protein 1 (Mcrip1), with C-terminal MYC/DDK tag, expressed in HEK293T cells.

Specification

• Description: The phosphorylation status of MCRIP1 functions as a molecular switch to regulate epithelial-mesenchymal transition. Unphosphorylated MCRIP1 binds to and inhibits the transcriptional corepressor CTBP(s). When phosphorylated by MAPK/ERK, MCRIP1 releases CTBP(s) resulting in transcriptional silencing of the E-cadherin gene and induction of epithelial-mesenchymal transition.
• Source: HEK293T
• Species: Mouse
• Tag: Myc/DDK
• Molecular Mass: 11.1 kDa
• Purity: > 80% as determined by SDS-PAGE and Coomassie blue staining
• Stability: Stable for 12 months from the date of receipt of the product under proper storage and handling conditions. Avoid repeated freeze-thaw cycles.
• Storage: Store at -80 centigrade after receiving vials.
• Concentration: >50 μg/mL as determined by microplate BCA method
• Storage Buffer: 25 mM Tris.HCl, pH 7.3, 100 mM glycine, 10% glycerol.

Gene Information

• Gene Name: Mcrip1 MAPK regulated corepressor interacting protein 1 [ Mus musculus (house mouse) ]
• Official Symbol: Mcrip1
• Synonyms: Mcrip1; MAPK regulated corepressor interacting protein 1; Fam195; Fam195b; mapk-regulated corepressor-interacting protein 1; family with sequence similarity 195, member B; protein FAM195B
• Gene ID: 192173
• mRNA Refseq: NM_001033231
• Protein Refseq: NP_001028403
• UniProt ID: Q3UGS4