| Description : |
E3 ubiquitin-protein ligase that mediates ubiquitination of p53/TP53, leading to its degradation by the proteasome. Inhibits p53/TP53- and p73/TP73-mediated cell cycle arrest and apoptosis by binding its transcriptional activation domain. Also acts as a ubiquitin ligase E3 toward itself and ARRB1. Permits the nuclear export of p53/TP53. Promotes proteasome-dependent ubiquitin-independent degradation of retinoblastoma RB1 protein. Inhibits DAXX-mediated apoptosis by inducing its ubiquitination and degradation. Component of the TRIM28/KAP1-MDM2-p53/TP53 complex involved in stabilizing p53/TP53. Also component of the TRIM28/KAP1-ERBB4-MDM2 complex which links growth factor and DNA damage response pathways. Mediates ubiquitination and subsequent proteasome degradation of DYRK2 in nucleus. Ubiquitinates IGF1R and SNAI1 and promotes th to proteasomal degradation . |
| Source : |
Yeast |
| Species : |
Mouse |
| Tag : |
His |
| Form : |
Tris-based buffer, 50% glycerol. |
| Molecular Mass : |
56.6kDa |
| Protein length : |
1-489aa |
| AA Sequence : |
MCNTNMSVSTEGAASTSQIPASEQE TLVRPKPLLLKLLKSVGAQNDTYTM KEIIFYIGQYIMTKRLYDEKQQHIV YCSNDLLGDVFGVPSFSVKEHRKIY AMIYRNLVAVSQQDSGTSLSESRRQ PEGGSDLKDPLQAPPEEKPSSSDLI SRLSTSSRRRSISETEENTDELPGE RHRKRRRSLSFDPSLGLCELREMCS GGSSSSSSSSSESTETPSHQDLDDG VSEHSGDCLDQDSVSDQFSVEFEVE SLDSEDYSLSDEGHELSDEDDEVYR VTVYQTGESDTDSFEGDPEISLADY WKCTSCNEMNPPLPSHCKRCWTLRE NWLPDDKGKDKVEISEKAKLENSAQ AEEGLDVPDGKKLTENDAKEPCAEE DSEEKAEQTPLSQESDDYSQPSTSS SIVYSSQESVKELKEETQDKDESVE SSFSLNAIEPCVICQGRPKNGCIVH GKTGHLMSCFTCAKKLKKRNKPCPV CRQPIQMIVLTYFN |
| Purity : |
Greater than 90% as determined by SDS-PAGE. |
| Notes : |
Repeated freezing and thawing is not recommended. Store working aliquots at 4 centigrade for up to one week. |
| Storage : |
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself. Generally, the shelf life of liquid form is 6 months at -20 centigrade/-80 centigrade. The shelf life of lyophilized form is 12 months at -20 centigrade/-80 centigrade. |
