Recombinant Mouse Mepce Protein, Myc/DDK-tagged

• Cat.No.: Mepce-4034M
• Product Overview: Purified recombinant protein of mouse full-length methylphosphate capping enzyme (Mepce), with C-terminal MYC/DDK tag, expressed in HEK293T cells.

Specification

• Description: S-adenosyl-L-methionine-dependent methyltransferase that adds a methylphosphate cap at the 5'-end of 7SK snRNA (7SK RNA), leading to stabilize it. Also has a non-enzymatic function as part of the 7SK RNP complex: the 7SK RNP complex sequesters the positive transcription elongation factor b (P-TEFb) in a large inactive 7SK RNP complex preventing RNA polymerase II phosphorylation and subsequent transcriptional elongation. The 7SK RNP complex also promotes snRNA gene transcription by RNA polymerase II via interaction with the little elongation complex (LEC). In the 7SK RNP complex, MEPCE is required to stabilize 7SK RNA and facilitate the assembly of 7SK RNP complex. MEPCE has a non-enzymatic function in the 7SK RNP complex; it has a non-enzymatic function; interaction with LARP7 within the 7SK RNP complex occluding its catalytic center.
• Source: HEK293T
• Species: Mouse
• Tag: Myc/DDK
• Molecular Mass: 72.5 kDa
• Purity: > 80% as determined by SDS-PAGE and Coomassie blue staining
• Stability: Stable for 12 months from the date of receipt of the product under proper storage and handling conditions. Avoid repeated freeze-thaw cycles.
• Storage: Store at -80 centigrade after receiving vials.
• Concentration: >50 μg/mL as determined by microplate BCA method
• Storage Buffer: 25 mM Tris.HCl, pH 7.3, 100 mM glycine, 10% glycerol.

Gene Information

• Gene Name: Mepce methylphosphate capping enzyme [ Mus musculus (house mouse) ]
• Official Symbol: Mepce
• Synonyms: MEPCE; methylphosphate capping enzyme; 7SK snRNA methylphosphate capping enzyme; bin3, bicoid-interacting 3, homolog; Bcdin3; D5Wsu46e; MGC28888; MGC30590
• Gene ID: 231803
• mRNA Refseq: NM_144913
• Protein Refseq: NP_659162
• UniProt ID: Q8K3A9