Recombinant Mouse Mical1 Protein, Myc/DDK-tagged

• Cat.No.: Mical1-4065M
• Product Overview: Purified recombinant protein of mouse full-length microtubule associated monooxygenase, calponin and LIM domain containing 1 (Mical1), with C-terminal MYC/DDK tag, expressed in HEK293T cells.

Specification

• Description: Monooxygenase that promotes depolymerization of F-actin by mediating oxidation of specific methionine residues on actin to form methionine-sulfoxide, resulting in actin filament disassembly and preventing repolymerization. In the absence of actin, it also functions as a NADPH oxidase producing H2O2. Acts as a cytoskeletal regulator that connects NEDD9 to intermediate filaments. Also acts as a negative regulator of apoptosis via its interaction with STK38 and STK38L; acts by antagonizing STK38 and STK38L activation by MST1/STK4. Involved in regulation of lamina-specific connectivity in the nervous system such as the development of lamina-restricted hippocampal connections. Through redox regulation of the actin cytoskeleton controls the intracellular distribution of secretory vesicles containing L1/neurofascin/NgCAM family proteins in neurons, thereby regulating their cell surface levels. May act as Rab effector protein and play a role in vesicle trafficking.
• Source: HEK293T
• Species: Mouse
• Tag: Myc/DDK
• Molecular Mass: 116.8 kDa
• Purity: > 80% as determined by SDS-PAGE and Coomassie blue staining
• Stability: Stable for 12 months from the date of receipt of the product under proper storage and handling conditions. Avoid repeated freeze-thaw cycles.
• Storage: Store at -80 centigrade after receiving vials.
• Concentration: >50 μg/mL as determined by microplate BCA method
• Storage Buffer: 25 mM Tris.HCl, pH 7.3, 100 mM glycine, 10% glycerol.

Gene Information

• Gene Name: Mical1 microtubule associated monooxygenase, calponin and LIM domain containing 1 [ Mus musculus (house mouse) ]
• Official Symbol: Mical1
• Synonyms: MICAL1; microtubule associated monoxygenase, calponin and LIM domain containing 1; protein-methionine sulfoxide oxidase MICAL1; CasL interacting molecule MICAL; molecule interacting with CasL protein 1; NEDD9 interacting protein with calponin homology and LIM domains; NEDD9-interacting protein with calponin homology and LIM domains; MICAL; Nical; MICAL-1; MGC27831; MGC38321
• Gene ID: 171580
• mRNA Refseq: NM_138315
• Protein Refseq: NP_612188
• UniProt ID: Q8VDP3