Recombinant Mouse Mitf Protein, His&GST-tagged

• Cat.No.: Mitf-1779M
• Product Overview: Recombinant Mouse Mitf Protein (Asn180-Gly290) with N-His&GST tag was expressed in E. coli.

Specification

• Description: This transcription factor serves at a critical point between extracellular signaling and downstream targets in cell specification in early eye and neural crest development. Mutant alleles have been identified that generate distinct phenotypes. Some of these alleles are being used to model the human diseases Waardenburg syndrome IIa and Tietz syndrome.
• Source: E. coli
• Species: Mouse
• Tag: N-His&GST
• Form: Freeze-dried powder
• Molecular Mass: Predicted Molecular Mass: 43.4 kDa
  Accurate Molecular Mass: 44 kDa
• Protein length: Asn180-Gly290
• Purity: > 90%
• Applications: Positive Control; Immunogen; SDS-PAGE; WB.
• Stability: The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37 centigrade for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.
• Storage: Avoid repeated freeze/thaw cycles. Store at 2-8 centigrade for one month. Aliquot and store at -80 centigrade for 12 months.
• Storage Buffer: 20mM Tris, 150mM NaCl, pH8.0, containing 1mM EDTA, 1mM DTT, 0.01% SKL, 5% Trehalose and Proclin300.
• Reconstitution: Reconstitute in 20mM Tris, 150mM NaCl (pH8.0) to a concentration of 0.1-1.0 mg/mL. Do not vortex.

Gene Information

• Gene Name: Mitf microphthalmia-associated transcription factor [ Mus musculus (house mouse) ]
• Official Symbol: Mitf
• Synonyms: MITF; microphthalmia-associated transcription factor; black eyed white; Wh; bw; mi; vit; Bhlhe32; Vitiligo; MGC124309; MGC124310
• Gene ID: 17342
• mRNA Refseq: NM_001113198
• Protein Refseq: NP_001106669
• UniProt ID: Q08874