Recombinant Mouse Mus81 Protein, Myc/DDK-tagged

• Cat.No.: Mus81-4226M
• Product Overview: Purified recombinant protein of mouse full-length MUS81 structure-specific endonuclease subunit (Mus81), with C-terminal MYC/DDK tag, expressed in HEK293T cells.

Specification

• Species: Mouse
• Source: HEK293
• Tag: DDK&Myc
• Description: Interacts with EME1 and EME2 to form a DNA structure-specific endonuclease with substrate preference for branched DNA structures with a 5'-end at the branch nick. Typical substrates include 3'-flap structures, replication forks and nicked Holliday junctions. May be required in mitosis for the processing of stalled or collapsed replication forks.
• Molecular Mass: 62 kDa
• Purity: > 80% as determined by SDS-PAGE and Coomassie blue staining
• Stability: Stable for 12 months from the date of receipt of the product under proper storage and handling conditions. Avoid repeated freeze-thaw cycles.
• Storage: Store at -80 centigrade after receiving vials.
• Concentration: >50 μg/mL as determined by microplate BCA method
• Storage Buffer: 25 mM Tris.HCl, pH 7.3, 100 mM glycine, 10% glycerol.

Gene Information

• Gene Name: Mus81 MUS81 structure-specific endonuclease subunit [ Mus musculus (house mouse) ]
• Official Symbol: Mus81
• Synonyms: MUS81; MUS81 endonuclease homolog (yeast); crossover junction endonuclease MUS81; AI182501; AW045863; 1200008A18Rik
• Gene ID: 71711
• mRNA Refseq: NM_027877
• Protein Refseq: NP_082153
• UniProt ID: Q91ZJ0