Active Recombinant Mouse NEK3 Protein, GST & His-tagged

• Cat.No.: NEK3-924M
• Product Overview: Recombinant mouse NEK3 (ABK42288.1) (Met 1-Ala 509) was fused with the N-terminal polyhistidine-tagged GST tag at the N-terminus.

Specification

• Source: Baculovirus-Insect Cells
• Species: Mouse
• Tag: GST & His
• Predicted N Terminal: Met
• Form: Supplied as sterile 20mM Tris, 500mM NaCl, pH 8.0, 10% glycerol.
• Bio-activity: The specific activity was determined to be 8 nmol/min/mg using MBP as substrate.
• Molecular Mass: The secreted recombinant mouse NEK3/GST chimera consists of 746 amino acids and has a calculated molecular mass of 84.8 kDa. The recombinant protein migrates as an approximately 72 kDa band in SDS-PAGE under reducing conditions.
• Endotoxin: < 1.0 EU per μg of the protein as determined by the LAL method.
• Purity: >82 % as determined by SDS-PAGE.
• Stability: Samples are stable for up to twelve months from date of receipt at -70ºC.
• Storage: Store it under sterile conditions at -20ºC~-70ºC. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.

Gene Information

• Gene Name: Nek3 NIMA (never in mitosis gene a)-related expressed kinase 3 [ Mus musculus ]
• Official Symbol: NEK3
• Synonyms: NEK3; NIMA (never in mitosis gene a)-related expressed kinase 3; serine/threonine-protein kinase Nek3; nimA-related protein kinase 3; NIMA-related expressed kinase 3; never in mitosis A-related kinase 3
• Gene ID: 23954
• mRNA Refseq: NM_001162947
• Protein Refseq: NP_001156419
• Function: ATP binding; kinase activity; metal ion binding; nucleotide binding; protein kinase activity; protein serine/threonine kinase activity; transferase activity; transferase activity, transferring phosphorus-containing groups