Recombinant Mouse Netrin 1 Protein, Flag tagged

• Cat.No.: Ntn1-017H
• Product Overview: The extracellular domain of mouse netrin-1 (aa 27-604) fused at the C-terminus to a FLAG-tag was produced in HEK 293 cells.

Specification

• Species: Mouse
• Source: HEK293
• Tag: Flag
• Protein Length: 27-604 aa
• Description: Predicted to be an extracellular matrix structural constituent. Involved in chemorepulsion of axon and regulation of synapse assembly. Acts upstream of or within several processes, including generation of neurons; inner ear morphogenesis; and regulation of neurogenesis. Located in basement membrane and cytoplasm. Is active in glutamatergic synapse. Is expressed in several structures, including alimentary system; central nervous system; integumental system; lung; and sensory organ. Human ortholog(s) of this gene implicated in congenital mirror movement disorder. Orthologous to human NTN1 (netrin 1).
• Tag: C-Flag
• Molecular Mass: ~80 kDa
• Endotoxin: < 0.10 EU/μg of the protein by the LAL method.
• Purity: ≥90% (SDS-PAGE), Purified by multi-step chromatography.
• Specificity: Binds to human and mouse UNC5B.
• Applications: ELISA: binds to UNC5B.
• Stability: Stable for at least 6 months after receipt when stored at -20 centigrade.
• Reconstitution: Reconstitute with 100 μL sterile distilled water. Resuspended protein should be used immediately upon reconstitution to avoid loss in activity due to non-specific binding to the vial. Further dilutions should be made with medium containing 5% fetal calf serum or 0.5% BSA.
• Storage: Avoid freeze/thaw cycles. After reconstitution, prepare aliquots and store at -20 centigrade.
  Long Term Storage: -20 centigrade
• Concentration: 0.1 mg/mL after reconstitution.
• Storage Buffer: Lyophilized. Contains PBS.
• Shipping: Blue Ice

Gene Information

• Gene Name: Ntn1 netrin 1 [ Mus musculus (house mouse) ]
• Official Symbol: Ntn1
• Synonyms: NTN1; netrin 1; netrin-1; AI561871; BC019633; Netrin-1
• Gene ID: 18208
• mRNA Refseq: NM_008744
• Protein Refseq: NP_032770
• UniProt ID: O09118