Recombinant Mouse NFE2L2 protein, His-tagged

• Cat.No.: NFE2L2-125M
• Product Overview: Recombinant Mouse NFE2L2 protein is produced by E.coli expression system and the residues Ala375-Asn597 is expressed with a His tag at the N-terminal.

Specification

• Source: E. coli
• Species: Mouse
• Tag: His
• Form: 100 mM NaHCO3, 500 mM NaCl, pH 8.3, containing 1 mM EDTA, 1 mM DTT, 0.01 % SKL, 5 % Trehalose and Proclin 300.
• Molecular Mass: 34 kDa
• Protein length: Ala375-Asn597
• AA Sequence: APGSVKQNGPKAQPAHSPGDTVQPL SPAQGHSAPMRESQCENTTKKEVPV SPGHQKAPFTKDKHSSRLEAHLTRD ELRAKALHIPFPVEKIINLPVDDFN EMMSKEQFNEAQLALIRDIRRRGKN KVAAQNCRKRKLENIVELEQDLGHL KDEREKLLREKGENDRNLHLLKRRL STLYLEVFSMLRDEDGKPYSPSEYS LQQTRDGNVFLVPKSKKPDTKKN
• Endotoxin: Less than 1.0 EU per ug by the LAL method.
• Purity: > 95 %
• Applications: Positive Control; Immunogen; SDS-PAGE; WB.
• Stability: The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37 centigrade for 48 h, and no obvious degradation and precipitation were observed. The loss rate is less than 5 % within the expiration date under appropriate storage condition.
• Storage: Avoid repeated freeze/thaw cycles. Store at 2-8 centigrade for one month. Aliquot and store at -80 centigrade for 12 months.
• Storage Buffer: 100 mM NaHCO3, 500 mM NaCl, pH 8.3, containing 1 mM EDTA, 1 mM DTT, 0.01 % SKL, 5 % Trehalose and Proclin 300.
• Reconstitution: Reconstitute in 100 mM NaHCO3, 500 mM NaCl (pH 8.3) to a concentration of 0.1-1.0 mg/mL. Do not vortex.

Gene Information

• Gene Name: NFE2L2 nuclear factor (erythroid-derived 2)-like 2 [ Homo sapiens ]
• Official Symbol: NFE2L2
• Synonyms: NRF2; HEBP1; NF-E2-Related Factor 2; Nuclear factor, erythroid derived 2, like 2
• Gene ID: 4780
• mRNA Refseq: NM_001145412
• Protein Refseq: NP_001138884
• MIM: 600492
• UniProt ID: Q16236

Reviews

03/01/2021

The NFE2L2 proteins purchased from your company is suitable for ELISA.

12/04/2020

The technician modified my experimental protocol so that my experiment went smoothly.

11/21/2020

Very good after-sales service to get my experiments going!