Recombinant Mouse Ngb Protein, Myc/DDK-tagged

• Cat.No.: Ngb-4404M
• Product Overview: Purified recombinant protein of mouse full-length neuroglobin (Ngb), with C-terminal MYC/DDK tag, expressed in HEK293T cells.

Specification

• Description: Involved in oxygen transport in the brain. Hexacoordinate globin, displaying competitive binding of oxygen or the distal His residue to the iron atom. Not capable of penetrating cell membranes. The deoxygenated form exhibits nitrite reductase activity inhibiting cellular respiration via NO-binding to cytochrome c oxidase. Involved in neuroprotection during oxidative stress. May exert its anti-apoptotic activity by acting to reset the trigger level of mitochondrial cytochrome c release necessary to commit the cells to apoptosis.
• Source: HEK293T
• Species: Mouse
• Tag: Myc/DDK
• Molecular Mass: 17 kDa
• Purity: > 80% as determined by SDS-PAGE and Coomassie blue staining
• Stability: Stable for 12 months from the date of receipt of the product under proper storage and handling conditions. Avoid repeated freeze-thaw cycles.
• Storage: Store at -80 centigrade after receiving vials.
• Concentration: >50 μg/mL as determined by microplate BCA method
• Storage Buffer: 25 mM Tris.HCl, pH 7.3, 100 mM glycine, 10% glycerol.

Gene Information

• Gene Name: Ngb neuroglobin [ Mus musculus (house mouse) ]
• Official Symbol: Ngb
• Synonyms: NGB; neuroglobin
• Gene ID: 64242
• mRNA Refseq: NM_022414
• Protein Refseq: NP_071859
• UniProt ID: Q9ER97