Active Recombinant Mouse Nlrp3 protein, His-tagged

• Cat.No.: Nlrp3-1748M
• Product Overview: Active Recombinant Mouse Nlrp3 protein(T1W2H6)(671-1033 aa), fused with N-terminal His tag, was expressed in E.coli.

Specification

• Species: Mouse
• Source: E.coli
• Tag: His
• Protein Length: 671-1033 aa
• Form: PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
• Bio-activity: NLR Family, Pyrin Domain Containing Protein 3 (NLRP3), also known as NALP3, CIAS1, PYPAF or Cryopyrin, is a cytosolic multiprotein complex composed of the innate immune receptor protein NLRP3, adapter protein ASC, and inflammatory protease caspase-1 that responds to microbial infection, endogenous danger signals, and environmental stimuli. Aberrant activation of the NLRP3 inflammasome is associated with the pathogenesis of various inflammatory diseases, such as diabetes, cancer, and Alzheimer‘s disease. Interleukin 1 Beta (IL1b) can interact with NLRP3, thus a functional binding ELISA assay was conducted to detect the interaction of recombinant mouse NLRP3 and recombinant chicken IL1b. Briefly, NLRP3 was diluted serially in PBS with 0.01% BSA (pH 7.4). Duplicate samples of 100 μl were then transferred to IL1b-coated microtiter wells and incubated for 1h at 37°C. Wells were washed with PBST and incubated for 1h with anti-NLRP3 pAb, then aspirated and washed 3 times. After incubation with HRP labelled secondary antibody for 1h at 37°C, wells were aspirated and washed 5 times. With the addition of substrate solution, wells were incubated 15-25 minutes at 37°C. Finally, add 50 µL stop solution to the wells and read at 450/630 nm immediately. The binding activity of recombinant mouse NLRP3 and recombinant chicken IL1b was shown, the EC50 for this effect is 0.16 ug/mL.
• Molecular Mass: 43.9kDa
• Endotoxin: <1.0EU per 1μg (determined by the LAL method).
• Purity: > 95%
• Storage: Avoid repeated freeze/thaw cycles. Store at 2-8°C for one month. Store at -20°C for 12 months. Aliquot and store at -80°C for 12 months.
• Reconstitution: Reconstitute in 10mM PBS (pH7.4) to a concentration of 0.1-1.0 mg/mL. Do not vortex.

Gene Information

• Gene Name: Nlrp3 NLR family, pyrin domain containing 3 [ Mus musculus ]
• Official Symbol: Nlrp3
• Synonyms: NLRP3; NLR family, pyrin domain containing 3; NACHT, LRR and PYD domains-containing protein 3; cryopyrin; PYRIN-containing APAF1-like protein 1; cold autoinflammatory syndrome 1 homolog; mast cell maturation inducible protein 1; NACHT/LRR/pyrin domain-containing protein 3; cold autoinflammatory syndrome 1 protein homolog; mast cell maturation-associated-inducible protein 1; FCU; MWS; FCAS; Cias1; Mmig1; NALP3; Pypaf1; AII/AVP; AGTAVPRL; MGC129375
• Gene ID: 216799
• mRNA Refseq: NM_145827
• Protein Refseq: NP_665826