Recombinant Mouse Nlrp3 protein, His-tagged

• Cat.No.: Nlrp3-1748M
• Product Overview: Recombinant Mouse Nlrp3 aa. (Cys671~Trp1033) fused with N-terminal His tag was produced in E. coli cells.

Specification

• Species: Mouse
• Source: E.coli
• Tag: His
• Protein Length: Cys671~Trp1033
• Form: Freeze-dried powder
• Molecular Mass: 42kDa as determined by SDS-PAGE reducing conditions.
• Endotoxin: <1.0EU per 1ug (determined by the LAL method)
• Purity: >95%
• Applications: Positive Control; Immunogen; SDS-PAGE; WB.
• Stability: The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.
• Storage: Avoid repeated freeze/thaw cycles. Store at 2-8°C for one month. Aliquot and store at -80°C for 12 months.
• Concentration: 200μg/mL
• Storage buffer: 20mM Tris, 150mM NaCl, pH8.0, containing 1mM EDTA, 1mM DTT, 0.01% sarcosyl, 5%Trehalose and Proclin300.
• Reconstitution: Reconstitute in 20mM Tris, 150mM NaCl (pH8.0) to a concentration of 0.1-1.0 mg/mL. Do not vortex.
• Isoelectric Point: 6.1

Gene Information

• Gene Name: Nlrp3 NLR family, pyrin domain containing 3 [ Mus musculus (house mouse) ]
• Official Symbol: Nlrp3
• Synonyms: Nlrp3; NLR family, pyrin domain containing 3; FCU; MWS; FCAS; Cias1; Mmig1; NALP3; Pypaf1; AII/AVP; AGTAVPRL; NACHT, LRR and PYD domains-containing protein 3; NACHT/LRR/pyrin domain-containing protein 3; PYRIN-containing APAF1-like protein 1; cold autoinflammatory syndrome 1 homolog; cold autoinflammatory syndrome 1 protein homolog; cryopyrin; mast cell maturation inducible protein 1; mast cell maturation-associated-inducible protein 1
• Gene ID: 216799
• mRNA Refseq: NM_145827.3
• Protein Refseq: NP_665826.1
• UniProt ID: Q8R4B8