Recombinant Mouse Nmnat2 Protein, Myc/DDK-tagged

• Cat.No.: Nmnat2-4444M
• Product Overview: Purified recombinant protein of mouse full-length nicotinamide nucleotide adenylyltransferase 2 (Nmnat2), with C-terminal MYC/DDK tag, expressed in HEK293T cells.

Specification

• Description: Nicotinamide/nicotinate-nucleotide adenylyltransferase that acts as an axon maintenance factor. Catalyzes the formation of NAD+ from nicotinamide mononucleotide (NMN) and ATP. Can also use the deamidated form; nicotinic acid mononucleotide (NaMN) as substrate but with a lower efficiency. Cannot use triazofurin monophosphate (TrMP) as substrate. Also catalyzes the reverse reaction, i.e. the pyrophosphorolytic cleavage of NAD+. For the pyrophosphorolytic activity prefers NAD+, NADH and NaAD as substrates and degrades nicotinic acid adenine dinucleotide phosphate (NHD) less effectively. Fails to cleave phosphorylated dinucleotides NADP+, NADPH and NaADP+. Axon survival factor required for the maintenance of healthy axons: acts by delaying Wallerian axon degeneration, an evolutionarily conserved process that drives the loss of damaged axons.
• Source: HEK293T
• Species: Mouse
• Tag: Myc/DDK
• Molecular Mass: 35 kDa
• Purity: > 80% as determined by SDS-PAGE and Coomassie blue staining
• Stability: Stable for 12 months from the date of receipt of the product under proper storage and handling conditions. Avoid repeated freeze-thaw cycles.
• Storage: Store at -80 centigrade after receiving vials.
• Concentration: >50 μg/mL as determined by microplate BCA method
• Storage Buffer: 25 mM Tris.HCl, pH 7.3, 100 mM glycine, 10% glycerol.

Gene Information

• Gene Name: Nmnat2 nicotinamide nucleotide adenylyltransferase 2 [ Mus musculus (house mouse) ]
• Official Symbol: Nmnat2
• Synonyms: NMNAT2; nicotinamide nucleotide adenylyltransferase 2; nicotinamide mononucleotide adenylyltransferase 2; NMN adenylyltransferase 2; naMN adenylyltransferase 1; pyridine nucleotide adenylyltransferase 2; nicotinate-nucleotide adenylyltransferase 1; PNAT1; PNAT2; AI843915; D030041I09Rik; MGC113818
• Gene ID: 226518
• mRNA Refseq: NM_175460
• Protein Refseq: NP_780669
• UniProt ID: Q8BNJ3