Recombinant Mouse Npl Protein, Myc/DDK-tagged

• Cat.No.: Npl-4472M
• Product Overview: Purified recombinant protein of mouse full-length N-acetylneuraminate pyruvate lyase (Npl), with C-terminal MYC/DDK tag, expressed in HEK293T cells.

Specification

• Species: Mouse
• Source: HEK293
• Tag: DDK&Myc
• Description: Catalyzes the cleavage of N-acetylneuraminic acid (sialic acid) to form pyruvate and N-acetylmannosamine via a Schiff base intermediate. It prevents sialic acids from being recycled and returning to the cell surface. Involved in the N-glycolylneuraminic acid (Neu5Gc) degradation pathway.
• Molecular Mass: 35.1 kDa
• Purity: > 80% as determined by SDS-PAGE and Coomassie blue staining
• Stability: Stable for 12 months from the date of receipt of the product under proper storage and handling conditions. Avoid repeated freeze-thaw cycles.
• Storage: Store at -80 centigrade after receiving vials.
• Concentration: >50 μg/mL as determined by microplate BCA method
• Storage Buffer: 25 mM Tris.HCl, pH 7.3, 100 mM glycine, 10% glycerol.

Gene Information

• Gene Name: Npl N-acetylneuraminate pyruvate lyase [ Mus musculus (house mouse) ]
• Official Symbol: Npl
• Synonyms: NPL; N-acetylneuraminate pyruvate lyase; N-acetylneuraminate lyase; NALase; sialate lyase; sialic acid lyase; sialic acid aldolase; sialate-pyruvate lyase; N-acetylneuraminic acid aldolase; N-acetylneuraminate pyruvate-lyase; 0610033B02Rik
• Gene ID: 74091
• mRNA Refseq: NM_028749
• Protein Refseq: NP_083025
• UniProt ID: Q9DCJ9