Recombinant Mouse Npnt Protein, His-tagged

• Cat.No.: Npnt-1849M
• Product Overview: Recombinant Mouse Npnt Protein (His420-Cys561) with N-His tag was expressed in E. coli.

Specification

• Species: Mouse
• Source: E.coli
• Tag: His
• Protein Length: His420-Cys561
• Description: Enables integrin binding activity. Involved in several processes, including cell-cell adhesion mediated by integrin; pilomotor reflex; and positive regulation of alkaline phosphatase activity. Acts upstream of or within several processes, including branching involved in ureteric bud morphogenesis; positive regulation of cell-substrate adhesion; and positive regulation of transforming growth factor beta receptor signaling pathway. Located in basement membrane; extracellular region; and smooth muscle contractile fiber. Is expressed in several structures, including alimentary system; brain; genitourinary system; respiratory system; and sensory organ. Orthologous to human NPNT (nephronectin).
• Form: Freeze-dried powder
• Molecular Mass: Predicted Molecular Mass: 19.2 kDa
  Accurate Molecular Mass: 19 kDa
• Purity: > 90%
• Applications: Positive Control; Immunogen; SDS-PAGE; WB.
• Stability: The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37 centigrade for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.
• Storage: Avoid repeated freeze/thaw cycles. Store at 2-8 centigrade for one month. Aliquot and store at -80 centigrade for 12 months.
• Storage Buffer: 20mM Tris, 150mM NaCl, pH8.0, containing 1mM EDTA, 1mM DTT, 0.01% SKL, 5% Trehalose and Proclin300.
• Reconstitution: Reconstitute in ddH2O to a concentration of 0.1-1.0 mg/mL. Do not vortex.

Gene Information

• Gene Name: Npnt nephronectin [ Mus musculus (house mouse) ]
• Official Symbol: Npnt
• Synonyms: NPNT; nephronectin; preosteoblast EGF-like repeat protein with MAM domain; Nctn; POEM; AA682063; AI314031; 1110009H02Rik
• Gene ID: 114249
• mRNA Refseq: NM_001029836
• Protein Refseq: NP_001025007
• UniProt ID: Q91V88