Recombinant Mouse Nudt12 Protein, Myc/DDK-tagged

• Cat.No.: Nudt12-4534M
• Product Overview: Purified recombinant protein of mouse full-length nudix (nucleoside diphosphate linked moiety X)-type motif 12 (cDNA clone MGC:68086 IMAGE:2646355), complete cds, with C-terminal MYC/DDK tag, expressed in HEK293T cells.

Specification

• Description: mRNA decapping enzyme that specifically removes the nicotinamide adenine dinucleotide (NAD) cap from a subset of mRNAs by hydrolyzing the diphosphate linkage to produce nicotinamide mononucleotide (NMN) and 5' monophosphate mRNA. The NAD-cap is present at the 5'-end of some RNAs; in contrast to the canonical N7 methylguanosine (m7G) cap, the NAD cap promotes mRNA decay. Preferentially acts on NAD-capped transcripts in response to nutrient stress. Also acts on free nicotinamide adenine dinucleotide molecules: hydrolyzes NAD(H) into NMN(H) and AMP, and NADPH into NMNH and 2',5'-ADP. May act to regulate the concentration of peroxisomal nicotinamide nucleotide cofactors required for oxidative metabolism in this organelle.
• Source: HEK293T
• Species: Mouse
• Tag: Myc/DDK
• Molecular Mass: 40.8 kDa
• Purity: > 80% as determined by SDS-PAGE and Coomassie blue staining
• Stability: Stable for 12 months from the date of receipt of the product under proper storage and handling conditions. Avoid repeated freeze-thaw cycles.
• Storage: Store at -80 centigrade after receiving vials.
• Concentration: >50 μg/mL as determined by microplate BCA method
• Storage Buffer: 25 mM Tris.HCl, pH 7.3, 100 mM glycine, 10% glycerol.

Gene Information

• Gene Name: Nudt12 nudix (nucleoside diphosphate linked moiety X)-type motif 12 [ Mus musculus (house mouse) ]
• Official Symbol: Nudt12
• Synonyms: Nudt12; nudix (nucleoside diphosphate linked moiety X)-type motif 12; 0610016O18Rik; NAD-capped RNA hydrolase NUDT12; NADH pyrophosphatase NUDT12; deNADding enzyme NUDT12; nucleoside diphosphate-linked moiety X motif 12; nudix (nucleoside diphosphnudix (nucleoside diphosphate linked moiety X)-type motif 12ate linked moiety X)-type motif 12; nudix motif 12; peroxisomal NADH pyrophosphatase NUDT12; EC 3.6.1.22
• Gene ID: 67993
• mRNA Refseq: NM_026497
• Protein Refseq: NP_080773
• UniProt ID: Q9DCN1