Recombinant Mouse Otub1 Protein, MYC/DDK-tagged

• Cat.No.: Otub1-323M
• Product Overview: Purified recombinant protein of full-length mouse OTU domain, ubiquitin aldehyde binding 1 (Otub1), with C-terminal MYC/DDK tag, expressed in HEK293T cells.

Specification

• Description: Hydrolase that can specifically remove compared to 'Lys-48'-linked conjugated ubiquitin from proteins and plays an important regulatory role at the level of protein turnover by preventing degradation. Regulator of T-cell anergy, a phenomenon that occurs when T-cells are rendered unresponsive to antigen rechallenge and no longer respond to their cognate antigen. Acts via its interaction with RNF128/GRAIL. Surprisingly, it regulates RNF128-mediated ubiquitination, but does not deubiquitinate polyubiquitinated RNF128. Deubiquitinates estrogen receptor alpha (ESR1). Mediates deubiquitination of 'Lys-48'-linked polyubiquitin chains, but not 'Lys-63'-linked polyubiquitin chains. Not able to cleave di-ubiquitin. Also capable of removing NEDD8 from NEDD8 conjugates, but with a much lower preference compared to 'Lys-48'-linked ubiquitin.
• Source: HEK293T
• Species: Mouse
• Tag: MYC/DDK
• Molecular Mass: 31.3 kDa
• Purity: >80%, as determined by SDS-PAGE and Coomassie blue staining
• Stability: Stable for 12 months from the date of receipt of the product under proper storage and handling conditions. Avoid repeated freeze-thaw cycles.
• Storage: Store at -80 centigrade after receiving vials.
• Concentration: >50 ug/mL as determined by microplate BCA method
• Storage Buffer: 25 mM Tris.HCl, pH 7.3, 100 mM glycine, 10% glycerol.

Gene Information

• Gene Name: Otub1 OTU domain, ubiquitin aldehyde binding 1 [ Mus musculus (house mouse) ]
• Official Symbol: Otub1
• Synonyms: Otub1; OTU domain, ubiquitin aldehyde binding 1; AI850305; ubiquitin thioesterase OTUB1; OTU domain-containing ubiquitin aldehyde-binding protein 1; deubiquitinating enzyme OTUB1; otubain 1; ubiquitin-specific-processing protease OTUB1; EC 3.4.19.12
• Gene ID: 107260
• mRNA Refseq: NM_134150
• Protein Refseq: NP_598911
• UniProt ID: Q7TQI3