Recombinant Mouse Pan3 Protein, Myc/DDK-tagged

• Cat.No.: Pan3-4667M
• Product Overview: Purified recombinant protein of mouse full-length PAN3 polyA specific ribonuclease subunit homolog (S, with C-terminal MYC/DDK tag, expressed in HEK293T cells.

Specification

• Description: Regulatory subunit of the poly(A)-nuclease (PAN) deadenylation complex, one of two cytoplasmic mRNA deadenylases involved in general and miRNA-mediated mRNA turnover. PAN specifically shortens poly(A) tails of RNA and the activity is stimulated by poly(A)-binding protein (PABP). PAN deadenylation is followed by rapid degradation of the shortened mRNA tails by the CCR4-NOT complex. Deadenylated mRNAs are then degraded by two alternative mechanisms, namely exosome-mediated 3'-5' exonucleolytic degradation, or deadenlyation-dependent mRNA decaping and subsequent 5'-3' exonucleolytic degradation by XRN1. PAN3 acts as a positive regulator for PAN activity, recruiting the catalytic subunit PAN2 to mRNA via its interaction with RNA and PABP, and to miRNA targets via its interaction with GW182 family proteins.
• Source: HEK293T
• Species: Mouse
• Tag: Myc/DDK
• Molecular Mass: 62.8 kDa
• Purity: > 80% as determined by SDS-PAGE and Coomassie blue staining
• Stability: Stable for 12 months from the date of receipt of the product under proper storage and handling conditions. Avoid repeated freeze-thaw cycles.
• Storage: Store at -80 centigrade after receiving vials.
• Concentration: >50 μg/mL as determined by microplate BCA method
• Storage Buffer: 25 mM Tris.HCl, pH 7.3, 100 mM glycine, 10% glycerol.

Gene Information

• Gene Name: Pan3 PAN3 poly(A) specific ribonuclease subunit [ Mus musculus (house mouse) ]
• Official Symbol: Pan3
• Synonyms: Pan3; PAN3 poly(A) specific ribonuclease subunit; AU014670; 2700050F09Rik; A430027N15Rik; PAN2-PAN3 deadenylation complex subunit Pan3; PAB-dependent poly(A)-specific ribonuclease subunit 3; PAB1P-dependent poly(A)-specific ribonuclease; PAN3 polyA specific ribonuclease subunit homolog; Pabp-dependent poly(A) nuclease 3; Pabp1-dependent poly A-specific ribonuclease subunit Pan3; poly(A)-nuclease deadenylation complex subunit 3; EC 3.1.13.4
• Gene ID: 72587
• mRNA Refseq: NM_028291
• Protein Refseq: NP_082567
• UniProt ID: Q640Q5