Recombinant Mouse Papss1 Protein, Myc/DDK-tagged

• Cat.No.: Papss1-4674M
• Product Overview: Purified recombinant protein of mouse full-length 3'-phosphoadenosine 5'-phosphosulfate synthase 1 (cDNA clone MGC:91299 IMAGE:6833756), complete cds, with C-terminal MYC/DDK tag, expressed in HEK293T cells.

Specification

• Description: Bifunctional enzyme with both ATP sulfurylase and APS kinase activity, which mediates two steps in the sulfate activation pathway. The first step is the transfer of a sulfate group to ATP to yield adenosine 5'-phosphosulfate (APS), and the second step is the transfer of a phosphate group from ATP to APS yielding 3'-phosphoadenylylsulfate (PAPS: activated sulfate donor used by sulfotransferase). In mammals, PAPS is the sole source of sulfate; APS appears to be only an intermediate in the sulfate-activation pathway. Required for normal biosynthesis of sulfated L-selectin ligands in endothelial cells.
• Source: HEK293T
• Species: Mouse
• Tag: Myc/DDK
• Molecular Mass: 68.5 kDa
• Purity: > 80% as determined by SDS-PAGE and Coomassie blue staining
• Stability: Stable for 12 months from the date of receipt of the product under proper storage and handling conditions. Avoid repeated freeze-thaw cycles.
• Storage: Store at -80 centigrade after receiving vials.
• Concentration: >50 μg/mL as determined by microplate BCA method
• Storage Buffer: 25 mM Tris.HCl, pH 7.3, 100 mM glycine, 10% glycerol.

Gene Information

• Gene Name: Papss1 3'-phosphoadenosine 5'-phosphosulfate synthase 1 [ Mus musculus (house mouse) ]
• Official Symbol: Papss1
• Synonyms: Papss1; 3'-phosphoadenosine 5'-phosphosulfate synthase 1; A; SK; SK1; Asapk; AI325286; bifunctional 3'-phosphoadenosine 5'-phosphosulfate synthase 1; PAPS synthase 1; PAPSS 1; SK 1; sulfurylase kinase 1; EC 2.7.1.25; EC 2.7.7.4
• Gene ID: 23971
• mRNA Refseq: NM_011863
• Protein Refseq: NP_035993
• UniProt ID: Q60967