Recombinant Mouse Pdk3 Protein, Myc/DDK-tagged

• Cat.No.: Pdk3-4768M
• Product Overview: Purified recombinant protein of mouse full-length pyruvate dehydrogenase kinase, isoenzyme 3 (Pdk3), with C-terminal MYC/DDK tag, expressed in HEK293T cells.

Specification

• Description: Inhibits pyruvate dehydrogenase activity by phosphorylation of the E1 subunit PDHA1, and thereby regulates glucose metabolism and aerobic respiration. Can also phosphorylate PDHA2. Decreases glucose utilization and increases fat metabolism in response to prolonged fasting, and as adaptation to a high-fat diet. Plays a role in glucose homeostasis and in maintaining normal blood glucose levels in function of nutrient levels and under starvation. Plays a role in the generation of reactive oxygen species.
• Source: HEK293T
• Species: Mouse
• Tag: Myc/DDK
• Molecular Mass: 47.9 kDa
• Purity: > 80% as determined by SDS-PAGE and Coomassie blue staining
• Stability: Stable for 12 months from the date of receipt of the product under proper storage and handling conditions. Avoid repeated freeze-thaw cycles.
• Storage: Store at -80 centigrade after receiving vials.
• Concentration: >50 μg/mL as determined by microplate BCA method
• Storage Buffer: 25 mM Tris.HCl, pH 7.3, 100 mM glycine, 10% glycerol.

Gene Information

• Gene Name: Pdk3 pyruvate dehydrogenase kinase, isoenzyme 3 [ Mus musculus (house mouse) ]
• Official Symbol: Pdk3
• Synonyms: PDK3; pyruvate dehydrogenase kinase, isoenzyme 3; pyruvate dehydrogenase kinase, isozyme 3; pyruvate dehydrogenase kinase 3; [Pyruvate dehydrogenase [lipoamide]] kinase isozyme 3, mitochondrial; AI035637; MGC6383; 2610001M10Rik
• Gene ID: 236900
• mRNA Refseq: NM_145630
• Protein Refseq: NP_663605
• UniProt ID: Q922H2