Recombinant Mouse Pdlim4 Protein, Myc/DDK-tagged

• Cat.No.: Pdlim4-4772M
• Product Overview: Purified recombinant protein of mouse full-length PDZ and LIM domain 4 (Pdlim4), with C-terminal MYC/DDK tag, expressed in HEK293T cells.

Specification

• Description: Suppresses SRC activation by recognizing and binding to active SRC and facilitating PTPN13-mediated dephosphorylation of SRC 'Tyr-419' leading to its inactivation. Inactivated SRC dissociates from this protein allowing the initiation of a new SRC inactivation cycle. Involved in reorganization of the actin cytoskeleton. In nonmuscle cells, binds to ACTN1 (alpha-actinin-1), increases the affinity of ACTN1 to F-actin (filamentous actin), and promotes formation of actin stress fibers. Involved in regulation of the synaptic AMPA receptor transport in dendritic spines of hippocampal pyramidal neurons directing the receptors toward an insertion at the postsynaptic membrane. Links endosomal surface-internalized GRIA1-containing AMPA receptors to the alpha-actinin/actin cytoskeleton. Increases AMPA receptor-mediated excitatory postsynaptic currents in neurons.
• Source: HEK293T
• Species: Mouse
• Tag: Myc/DDK
• Molecular Mass: 35.6 kDa
• Purity: > 80% as determined by SDS-PAGE and Coomassie blue staining
• Stability: Stable for 12 months from the date of receipt of the product under proper storage and handling conditions. Avoid repeated freeze-thaw cycles.
• Storage: Store at -80 centigrade after receiving vials.
• Concentration: >50 μg/mL as determined by microplate BCA method
• Storage Buffer: 25 mM Tris.HCl, pH 7.3, 100 mM glycine, 10% glycerol.

Gene Information

• Gene Name: Pdlim4 PDZ and LIM domain 4 [ Mus musculus (house mouse) ]
• Official Symbol: Pdlim4
• Synonyms: PDLIM4; PDZ and LIM domain 4; PDZ and LIM domain protein 4; LIM protein RIL; reversion induced LIM; reversion-induced LIM protein; Ril
• Gene ID: 30794
• mRNA Refseq: NM_019417
• Protein Refseq: NP_062290
• UniProt ID: P70271