Recombinant Mouse PGF protein(Met1-Pro158)

• Cat.No.: PGF-1036M
• Product Overview: Recombinant Mouse PGF (P49764) (Met1-Pro158) was fused with five amino acids (DDDDK) at the C-terminus was expressed and purified in HEK293.

Specification

• Source: HEK293
• Species: Mouse
• Tag: N/A
• Protein length: Met1-Pro158
• Form: Lyophilized from sterile PBS, pH 7.4. Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization.
• Bio-activity: Measured by its binding ability in a functional ELISA. Immobilized mouse PGF at 10 μg/mL (100ul/well) can bind human VEGFR1-His. The EC50 of human VEGFR1-His is 0.07-0.15 μg/mL.
• Molecular Mass: The recombinant mouse PGF consists of 146 amino acids and has a calculated molecular mass of 16.6 kDa. The recombinant protein migrates as an approximately 28 kDa band in SDS-PAGE under reducing conditions.
• Endotoxin: < 1.0 EU per μg of the protein as determined by the LAL method
• Purity: > 90 % as determined by SDS-PAGE
• Storage: Samples are stable for up to twelve months from date of receipt at -20°C to -80°C. Store it under sterile conditions at -20°C to -80°C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
• Reconstitution: It is recommended that sterile water be added to the vial to prepare a stock solution of 0.2 ug/ul. Centrifuge the vial at 4°C before opening to recover the entire contents.

Gene Information

• Gene Name: Pgf placental growth factor [ Mus musculus ]
• Official Symbol: PGF
• Synonyms: PGF; placental growth factor; placenta growth factor; PIGF; Plgf; AI854365
• Gene ID: 18654
• mRNA Refseq: NM_008827
• Protein Refseq: NP_032853