Recombinant Mouse Plpp3 Protein, Myc/DDK-tagged

• Cat.No.: Plpp3-4940M
• Product Overview: Purified recombinant protein of mouse full-length phospholipid phosphatase 3 (Plpp3), with C-terminal MYC/DDK tag, expressed in HEK293T cells.

Specification

• Description: Magnesium-independent phospholipid phosphatase of the plasma membrane that catalyzes the dephosphorylation of a variety of glycerolipid and sphingolipid phosphate esters including phosphatidate/PA, lysophosphatidate/LPA, diacylglycerol pyrophosphate/DGPP, sphingosine 1-phosphate/S1P and ceramide 1-phosphate/C1P. Also acts on N-oleoyl ethanolamine phosphate/N-(9Z-octadecenoyl)-ethanolamine phosphate, a potential physiological compound. Has both an extracellular and an intracellular phosphatase activity, allowing the hydrolysis and the cellular uptake of these bioactive lipid mediators from the milieu, regulating signal transduction in different cellular processes. Through the dephosphorylation of extracellular sphingosine-1-phosphate and the regulation of its extra- and intracellular availability, plays a role in vascular homeostasis, regulating endothelial cell migration, adhesion, survival, proliferation and the production of pro-inflammatory cytokines. By maintaining the appropriate levels of this lipid in the cerebellum, also ensure its proper development and function. Through its intracellular lipid phosphatase activity may act in early compartments of the secretory pathway, regulating the formation of Golgi to endoplasmic reticulum retrograde transport carriers.
• Source: HEK293T
• Species: Mouse
• Tag: Myc/DDK
• Molecular Mass: 35.2 kDa
• Purity: > 80% as determined by SDS-PAGE and Coomassie blue staining
• Stability: Stable for 12 months from the date of receipt of the product under proper storage and handling conditions. Avoid repeated freeze-thaw cycles.
• Storage: Store at -80 centigrade after receiving vials.
• Concentration: >50 μg/mL as determined by microplate BCA method
• Storage Buffer: 25 mM Tris.HCl, pH 7.3, 100 mM glycine, 10% glycerol.

Gene Information

• Gene Name: Plpp3 phospholipid phosphatase 3 [ Mus musculus (house mouse) ]
• Official Symbol: Plpp3
• Synonyms: Plpp3; phospholipid phosphatase 3; L; Pp; PRG; Lpp3; PRG-2; Ppab2b; Ppap2b; AV025606; D4Bwg0538e; D4Bwg1535e; 1110003O22Rik; 2610002D05Rik; phospholipid phosphatase 3; PAP-2b; PAP2-beta; PAP2b; lipid phosphate phosphohydrolase 3; phosphatidate phosphohydrolase type 2b; phosphatidic acid phosphatase 2b; phosphatidic acid phosphatase type 2B; EC 3.1.3.4
• Gene ID: 67916
• mRNA Refseq: NM_080555
• Protein Refseq: NP_542122
• UniProt ID: Q99JY8